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Updated: May 5, 2026

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
Published on: January 25, 2018
Isolation and sequence of cDNA encoding the soybean protease inhibitors PI IV and C-II
P E Joudrier1, D E Foard, L A Floener
1Department of Botany and Plant Pathology, Purdue University, 47907, West Lafayette, IN, USA.
Abstract:
Two full-length (or nearly so) cDNA clones containing information for the protease inhibitors PI IV and C-II from soybean seeds were identified by means of a synthetic probe. DNA sequencing revealed that the two protease inhibitors are synthesized as precursors with a short peptide leader. The coding regions of the two clones show 80% homology, wheraes the 5' non-coding regions are 90% homologous. Homology of 75% is found in the region extending beyond the stop codons.

