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Related Experiment Video

Updated: May 5, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Polyester μ-assay chip for stem cell studies.

Francesco Piraino¹, Seila Selimović, Marco Adamo

¹Bioengineering Department, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy ; Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, USA ; Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Biomicrofluidics

|November 27, 2013

Summary

This summary is machine-generated.

Researchers developed an easy-to-use microfluidic device for stem cell research. This platform enables high-throughput screening of growth factor effects on stem cell differentiation, maintaining pluripotency.

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Published on: November 26, 2018

Area of Science:

Biotechnology and Bioengineering
Stem Cell Biology
Microfluidics

Background:

Microfluidic technologies offer precise control over the cellular environment, reduced reagent use, and high-throughput screening for stem cell research.
A user-friendly microfluidic platform for studying growth factor effects on stem cell differentiation has been lacking.

Purpose of the Study:

To design and characterize a simple, easy-to-operate microfluidic device for studying stem cell differentiation.
To enable simultaneous screening of multiple stem cell culture conditions and growth factor concentrations.

Main Methods:

Fabrication of a polyester-based microfluidic chip using laser ablation and layer stacking.
Integration of a 10x10 microwell array, continuous perfusion system, and non-linear concentration gradient generator.
Numerical calculations for gradient formation and shear stress prediction; validation with murine embryonic stem cells.

Main Results:

The microfluidic device was successfully fabricated and characterized.
Murine embryonic stem cells cultured for 5 days maintained pluripotency across a range of leukemia inhibitory factor concentrations (0-1000 U/ml).

Conclusions:

The developed microfluidic device is simple, scalable, and fast to manufacture.
It provides a cell-compatible platform for long-term stem cell culture and differentiation studies.
This technology facilitates high-throughput screening of growth factors impacting stem cell behavior.