Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

1.3K
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
1.3K
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

1.9K
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
1.9K
Flame Photometry: Lab01:16

Flame Photometry: Lab

1.3K
In a flame photometer, when a solution like potassium chloride is aspirated into the flame, the solvent evaporates, leaving behind dehydrated salt. This salt dissociates into free gaseous atoms in their ground state. Some of these atoms absorb energy from the flame, leading to their excitation. The excited atoms return to the ground state, emitting photons at characteristic wavelengths. Because only electronic transitions are involved, the resulting emission lines are very narrow. The intensity...
1.3K
Atomic Absorption Spectroscopy: Lab01:21

Atomic Absorption Spectroscopy: Lab

1.3K
For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
 Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing...
1.3K
Atomic Spectroscopy: Absorption, Emission, and Fluorescence01:23

Atomic Spectroscopy: Absorption, Emission, and Fluorescence

3.2K
Atomic spectroscopy is a vital tool in elemental analysis, both qualitatively and quantitatively. It can be broadly divided into optical spectroscopy, mass spectroscopy, and X-ray spectroscopy methods. The optical spectroscopic methods are atomic absorption spectroscopy (AAS), atomic emission spectroscopy (AES), and atomic fluorescence spectroscopy (AFS). The first step in all three methods is atomization, where the solid, liquid, or solution-phase samples are converted into gas-phase atoms and...
3.2K
IR and UV–Vis Spectroscopy of Carboxylic Acids01:28

IR and UV–Vis Spectroscopy of Carboxylic Acids

4.6K
In IR spectroscopy of carboxylic acids, the C=O bond shows a characteristic band between 1710 and 1760 cm⁻¹, and the O–H bond exhibits a broad band between 2500 and 3300 cm⁻¹.
However, the stretching absorptions for the C=O bond vary depending on the structure of carboxylic acids. The C=O bond of the free carboxylic acids shows a higher stretching frequency, 1760 cm−1, while H-bonded carboxylic acids (dimers) exhibit stretching absorptions at a lower frequency,...
4.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Treatment Patterns of Goserelin 3.6 mg Once Every 4 Weeks and 10.8 mg Once Every 12 Weeks in Women With Breast Cancer: A Real-World Analysis of Patients in the United States.

JCO oncology practice·2025
Same author

Goserelin 3-month depot shows non-inferiority to the monthly formulation in U.S. patients with premenopausal breast cancer: a real-world evidence study.

Breast cancer research and treatment·2025
Same author

Isolation and characterization of Salmonella enteritidis bacteriophage Salmp-p7 isolated from slaughterhouse effluent and its application in food.

Archives of microbiology·2024
Same author

CT-Derived Features as Predictors of Clot Burden and Resolution.

Bioengineering (Basel, Switzerland)·2024
Same author

A generalized health index: automated thoracic CT-derived biomarkers predict life expectancy.

The British journal of radiology·2024
Same author

Comprehensive identification and characterization of simple sequence repeats based on the whole-genome sequences of 14 forest and fruit trees.

Forestry research·2024

Related Experiment Video

Updated: May 5, 2026

A New Straightforward Method for Lipophilicity logP Measurement using 19F NMR Spectroscopy
09:32

A New Straightforward Method for Lipophilicity logP Measurement using 19F NMR Spectroscopy

Published on: January 30, 2019

13.8K

Naphthenic acids quantification in organic solvents using fluorescence spectroscopy.

Nancy Martin1, Zvonko Burkus, Preston McEachern

  • 1a Department of Civil and Environmental Engineering , University of Alberta , Edmonton , Canada.

Journal of Environmental Science and Health. Part A, Toxic/Hazardous Substances & Environmental Engineering
|November 28, 2013
PubMed
Summary

This study introduces a cost-effective fluorescence method for quantifying naphthenic acids in water using organic solvents. Polar protic solvents, particularly methanol, offer superior sensitivity and linearity for improved water quality monitoring.

More Related Videos

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
09:46

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores

Published on: August 19, 2013

13.8K
Microwave-assisted Intramolecular Dehydrogenative Diels-Alder Reactions for the Synthesis of Functionalized Naphthalenes/Solvatochromic Dyes
12:07

Microwave-assisted Intramolecular Dehydrogenative Diels-Alder Reactions for the Synthesis of Functionalized Naphthalenes/Solvatochromic Dyes

Published on: April 1, 2013

15.5K

Related Experiment Videos

Last Updated: May 5, 2026

A New Straightforward Method for Lipophilicity logP Measurement using 19F NMR Spectroscopy
09:32

A New Straightforward Method for Lipophilicity logP Measurement using 19F NMR Spectroscopy

Published on: January 30, 2019

13.8K
Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
09:46

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores

Published on: August 19, 2013

13.8K
Microwave-assisted Intramolecular Dehydrogenative Diels-Alder Reactions for the Synthesis of Functionalized Naphthalenes/Solvatochromic Dyes
12:07

Microwave-assisted Intramolecular Dehydrogenative Diels-Alder Reactions for the Synthesis of Functionalized Naphthalenes/Solvatochromic Dyes

Published on: April 1, 2013

15.5K

Area of Science:

  • Environmental Chemistry
  • Analytical Chemistry

Background:

  • Traditional quantification of naphthenic acids is complex and costly.
  • Existing methods require organic solvent extraction and are unsuitable for preliminary research or continuous monitoring.

Purpose of the Study:

  • To evaluate fluorescence in organic solvents as an alternative for naphthenic acid quantification.
  • To assess the impact of different organic solvents on quantification accuracy and sensitivity.

Main Methods:

  • Tested nine organic solvents (polar protic, polar aprotic, non-polar).
  • Developed calibration curves and assessed light scattering and method sensitivity.
  • Optimized solvent mixtures and fluorescence parameters (synchronous mode, Δλ = 10 nm).

Main Results:

  • Polar protic solvents (methanol, ethanol, propanol) showed better performance with lower light scattering and higher sensitivity.
  • Methanol demonstrated strong linearity (R(2) > 0.99) below 250 mg/L and low relative standard deviation (< 10%).
  • A methanol-deionized water mixture (50:50) enhanced method sensitivity by 70%.

Conclusions:

  • Fluorescence in organic solvents is a viable, cost-effective alternative for naphthenic acid quantification.
  • Methanol-based solvent systems offer high sensitivity and linearity, suitable for water analysis.
  • The method is compatible with solid-phase extraction, enabling direct quantification.