Influence of cation markers on sulfate distribution in mouse gastrocnemius muscle

Further studies are reported which examines the influence of fixed negative sites on anion distribution in the interstitium of mouse gastrocnemius muscle. For this purpose, the 35S-sulfate distribution in in vitro muscles was examined in the absence and presence of a number of cations which are thought to bind to the interstitial fixed negative sites. Each of the cation treated muscles gave sulfate space measurements which were statistically greater than the control muscles. Ferritin, spermidine and ruthenium red treatment resulted in muscle sulfate spaces similar to the true morphometric ECV. This study suggests that the fixed negative sites in the interstitium of mouse gastrocnemius muscle exists in sufficient density to influence the distribution of anions in these tissue.