Decreased nucleic acid synthesis in low-dose-irradiated lymphocytes--effect of TCGF addition

Flow cytometry was used to evaluate nucleic acid synthesis in irradiated mixed lymphocyte cultures (MLC) compared to nonirradiated control cultures. Two staining methods were used (propidium iodide and acridine orange). We showed that RNA and DNA synthesis are retarded in MLC receiving 0.2 Gy. This effect was reversed by lymphocyte growth factor.