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Utilizing Time-Resolved Protein-Induced Fluorescence Enhancement to Identify Stable Local Conformations One α-Synuclein Monomer at a Time

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Deconstructing the photon stream from single nanocrystals: from binning to correlation.

Jian Cui¹, Andrew P Beyler, Thomas S Bischof

¹Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. mgb@mit.edu.

Chemical Society Reviews

|November 28, 2013

Summary

Single-molecule fluorescence spectroscopy reveals quantum dot physics. Photon correlation analysis of individual nanocrystals uncovers nanoscale electronic processes and dynamics.

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Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Published on: August 7, 2016

Area of Science:

* Nanoscience and Spectroscopy
* Quantum Dot Physics
* Materials Science

Background:

* Ensemble averaging previously obscured fundamental optical properties of colloidal semiconductor nanocrystal quantum dots.
* Inherent inhomogeneities in nanocrystals led to difficulties in understanding their behavior.
* Single quantum dot spectroscopy emerged as a crucial technique to overcome these limitations.

Purpose of the Study:

* To review methods for analyzing photon emission from single nanocrystals.
* To highlight the significance of photon correlation techniques in nanocrystal studies.
* To demonstrate how single-molecule spectroscopy reveals fundamental electronic processes.

Main Methods:

* Review of photon stream deconstruction paradigms from single nanocrystals.
* Comparison of intensity binning and single-photon counting methods.
* Emphasis on photon correlation as a powerful analytical tool.

Main Results:

* Single quantum dot spectroscopy resolves ambiguities in excitonic physics.
* Analysis of photon timing and energies reveals unexpected nanoscale electronic processes.
* Photon correlation techniques are advancing the study of multiexcitonic recombination dynamics and spectral properties.

Conclusions:

* Single-molecule spectroscopy is invaluable for understanding nanocrystal physics.
* Photon correlation enables direct extraction of single-nanocrystal properties from ensembles.
* These techniques offer unique insights into heterogeneous and dynamical systems.