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Identification of the Source of Secreted Proteins in the Kidney by Brefeldin A Injection
Published on: November 10, 2021
Circulating serum trefoil factor 3 (TFF3) is dramatically increased in chronic kidney disease
Ting-yi Du1, Hui-ming Luo, Hai-chun Qin
1Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan Province, China ; University of the Chinese Academy of Sciences, Beijing, China ; Department of Clinical Laboratory Medicine, the First People's Hospital of Yunnan Province, Kunming, Yunnan Province, China.
Serum trefoil factor 3 (TFF3) levels are elevated in chronic kidney disease (CKD) and correlate with disease severity. Understanding TFF3 in kidney disease is crucial for broader clinical research.
Area of Science:
- Biochemistry
- Clinical Medicine
- Nephrology
Background:
- Trefoil factor 3 (TFF3) is a peptide vital for mucosal integrity, cell growth, and migration.
- Aberrant TFF3 expression is linked to gastrointestinal inflammation, solid tumors, and other diseases.
Purpose of the Study:
- To investigate the distribution characteristics of serum TFF3 in various common clinical diseases.
- To explore the relationship between TFF3 concentrations and the severity of chronic kidney disease (CKD).
Main Methods:
- Enzyme-linked immunosorbent assay (ELISA) used to measure serum TFF3 in 1,072 Chinese patients.
- Matched case-control study for CKD stages 1-5.
- Immunohistochemistry (IHC) on renal tissues to assess TFF3 expression in relation to CKD severity.
Main Results:
- Mean serum TFF3 concentrations were significantly higher in patients with CKD, metastatic/secondary carcinoma (MC), and acute gastroenteritis (AG).
- Serum and urine TFF3 concentrations showed a positive correlation with CKD severity across stages 1-5.
- IHC confirmed TFF3 expression predominantly in renal tubular epithelial cells.
Conclusions:
- Clinical TFF3 research must account for the impact of kidney injury.
- Further investigation of TFF3 in CKD can elucidate its pathological roles in other diseases.
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