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Bacterial adherence to different meshes used in abdominal surgery
Ramon Pérez-Tanoira1, Maria-Carolina Isea-Peña, Angel Celdrán
11 Department of Microbiology, IIS-Fundación Jiménez Díaz , Madrid, Spain .
Background:
We studied the influence of morphology and type of material of abdominal wall prostheses in the avoidance of bacterial adhesion in acute and chronic mesh infections.
Methods:
Three different types of prostheses were compared: 1) High-density polypropylene monofilament mesh (PMM); 2) low-density PMM; and 3) prostheses composed of low-density polypropylene and a non-porous hydrophilic film (composite prostheses). Microbial adhesion tests were performed using reference strains of Staphylococcus aureus 15981, Staphylococcus epidermidis ATCC 35984, Mycobacterium abscessus DSM 44196, and Mycobacterium fortuitum ATCC 13756 using a protocol described previously.
Results:
Both Staphylococcus spp. and M. fortuitum strains showed lower adherence to PMM. Mycobacterium abscessus also exhibited lower adherence to composite prostheses. Both Mycobacterium spp. strains had lower adherence than Staphylococcus spp. strains for all materials except for low-density PMM. Mycobacterium fortuitum showed higher adherence to composite prostheses than M. abscessus, whereas the latter species had higher adherence to high-density PMM than M. fortuitum.
Conclusion:
Depending on the type of bacteria, collagen-coated hydrophilic prostheses with a large surface increased bacterial adherence significantly. These differences should be taken into consideration when choosing a mesh graft, which limits infection in abdominal wall reconstruction.
Insights
Mesh material and morphology impact bacterial adhesion in abdominal wall prostheses. Certain prostheses reduce adherence of Staphylococcus and Mycobacterium species, crucial for preventing mesh infections.
Area of Science:
- Biomaterials Science
- Infectious Diseases
- Surgical Innovation
Background:
- Abdominal wall prostheses are prone to bacterial adhesion, leading to acute and chronic infections.
- Understanding the influence of prosthesis material and morphology is key to preventing mesh-related complications.
Purpose of the Study:
- To evaluate the bacterial adhesion properties of different abdominal wall prostheses.
- To determine how material type and mesh structure affect bacterial colonization.
Main Methods:
- Three polypropylene monofilament mesh (PMM) types were tested: high-density, low-density, and composite (low-density PMM with a hydrophilic film).
- Microbial adhesion assays were conducted using reference strains of Staphylococcus aureus, Staphylococcus epidermidis, Mycobacterium abscessus, and Mycobacterium fortuitum.
Main Results:
- Polypropylene monofilament mesh (PMM) demonstrated lower adherence for Staphylococcus spp. and Mycobacterium fortuitum.
- Mycobacterium abscessus showed reduced adherence to composite prostheses.
- Mycobacterium spp. generally had lower adherence than Staphylococcus spp. on most materials, with exceptions noted for low-density PMM.
Conclusions:
- Bacterial adherence varies significantly based on prosthesis material, morphology, and the specific bacterial species.
- The choice of mesh graft is critical for minimizing infection risk in abdominal wall reconstruction, with specific materials showing differential efficacy against common pathogens.
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