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Desorption electrospray ionisation (DESI) for beginners--how to adjust settings for tissue imaging
Anna Bodzon-Kulakowska1, Anna Drabik, Joanna Ner
1Department of Biochemistry and Neurobiology, Faculty of Materials Sciences and Ceramics, AGH University of Science and Technology, Mickiewicza 30 ave, 30-059, Krakow, Poland.
Rapid Communications in Mass Spectrometry : RCM
|November 29, 2013
Summary
Optimizing desorption electrospray ionization (DESI) for biological samples is challenging. This study provides practical tips for ion source optimization, improving mass spectrometry imaging of tissues and other surfaces.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry Imaging
Background:
- Desorption electrospray ionization (DESI) is an ambient technique for surface imaging.
- Optimizing DESI, especially for biological samples, can be complex and discouraging.
Purpose of the Study:
- To provide practical tips and tricks for ion source optimization in DESI.
- To guide researchers in achieving desired results for biological and non-biological sample analysis.
Main Methods:
- Rat liver tissue and a rhodamine-marked surface were analyzed using a DESI ion source and mass spectrometer.
- Key parameters including ion source geometry, gas pressure, solvent flow, and capillary voltage were systematically varied.
Main Results:
- The impact of various parameters on mass spectral quality was demonstrated.
- Differences in optimization strategies for biological versus non-biological samples were highlighted.
Conclusions:
- Specific optimal parameters for analyzing biological tissues and non-biological surfaces using DESI were suggested.
- The study offers guidance for improving DESI performance and data quality.
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