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Middle region of FancM interacts with Mhf and Rmi1 in silkworms, a species lacking the Fanconi anaemia (FA) core

R Sugahara1, H Mon, J M Lee

  • 1Laboratory of Silkworm Science, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan.

Insect Molecular Biology
|November 30, 2013
PubMed

Insights

In silkworms lacking key Fanconi anemia (FA) proteins, FancM is crucial for DNA repair and cell survival after mitomycin C treatment. This study reveals FancM

Area of Science:

  • DNA repair mechanisms
  • Cellular response to genotoxic stress
  • Comparative genomics of DNA repair pathways

Background:

  • The Fanconi anemia (FA) pathway is essential for repairing DNA interstrand crosslinks (ICLs).
  • FANCM acts as a sensor and platform in the FA pathway, interacting with the Bloom's syndrome (BS) complex.
  • Silkworms (Bombyx mori) lack major FA core complex components, offering a unique model for studying FA pathway function.

Purpose of the Study:

  • To investigate the role of FancM in DNA repair and cell proliferation in silkworms, particularly in the context of mitomycin C (MMC) treatment.
  • To elucidate the molecular interactions and functional domains of silkworm FancM (BmFancM).

Main Methods:

  • Utilized silkworms (Bombyx mori) as a model organism lacking canonical FA core components.
  • Investigated the requirement of BmFancM for FancD2 monoubiquitination and cell proliferation under MMC exposure.
  • Analyzed BmFancM phosphorylation, subcellular localization, and interactions with Mhf1 and Rmi1 using biochemical assays.

Main Results:

  • BmFancM is essential for FancD2 monoubiquitination and cell proliferation in MMC-treated silkworms.
  • Phosphorylation of BmFancM influences its subcellular localization.
  • BmFancM interacts with Mhf1 and Rmi1, and this interaction is critical for MMC resistance, despite the absence of FAAP24.

Conclusions:

  • Silkworm FancM functions as a platform for recruiting both FA and BS proteins for DNA repair, even in the absence of canonical FA core components.
  • The findings highlight a conserved role for FancM in DNA interstrand crosslink repair, mediated by interactions with histone-fold proteins and BS complex subunits.
Keywords:
Bloom's pathwayDNA repairFanconi anaemia pathwayinsect

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