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Impedimetric aptasensor for ochratoxin A determination based on Au nanoparticles stabilized with hyper-branched
Gennady Evtugyn1, Anna Porfireva, Veronika Stepanova
1Analytical Chemistry Department, Kazan Federal University, 18 Kremlevskaya Street, Kazan 420008, Russian Federation. Tibor.Hianik@fmph.uniba.sk.
Sensors (Basel, Switzerland)
|November 30, 2013
Summary
A new impedimetric aptasensor detects ochratoxin A (OTA) in beverages. This sensor utilizes a gold electrode modified with Neutral Red, gold nanoparticles, and a dendrimeric polymer for sensitive and selective OTA detection.
Area of Science:
- Electrochemistry
- Biosensors
- Nanotechnology
Background:
- Ochratoxin A (OTA) is a prevalent mycotoxin found in various food products, posing significant health risks.
- Accurate and sensitive detection methods for OTA are crucial for food safety and quality control.
Purpose of the Study:
- To develop a novel impedimetric aptasensor for the sensitive and selective detection of ochratoxin A.
- To functionalize a gold electrode with a unique composite material for enhanced sensing performance.
Main Methods:
- Fabrication of a modified gold electrode using electropolymerized Neutral Red, gold nanoparticles, and a dendrimeric polymer (Botlorn H30®).
- Covalent attachment of thiolated aptamers specific to OTA onto the gold nanoparticles.
- Impedimetric measurements to quantify OTA based on changes in charge transfer resistance upon aptamer-OTA interaction.
Main Results:
- The aptasensor demonstrated a wide detection range for OTA from 0.1 to 100 nM, with a low limit of detection of 0.02 nM.
- High selectivity was achieved, with the sensor able to detect OTA in the presence of a 50-fold excess of ochratoxin B.
- Successful application in real samples, with good recovery rates (70% for light beer, 78% for dark beer) when testing spiked beer samples.
Conclusions:
- The developed impedimetric aptasensor offers a promising platform for the rapid and reliable detection of ochratoxin A.
- The novel electrode modification strategy enhances sensor performance, enabling sensitive and selective mycotoxin analysis.
- This aptasensor has potential applications in food safety monitoring, particularly for beverages like beer.
