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Polycaprolactone/oligomer compound scaffolds for cardiac tissue engineering.

Chaganti Srinivasa Reddy1, Jayarama Reddy Venugopal, Seeram Ramakrishna

  • 1Department of Mechanical Engineering, Technion-Israel Institute of Technology, Haifa, 32000, Israel; Institute of Biomaterial Science, Helmholtz-Zentrum Geesthacht, Kantstraße 55, Teltow, 14513, Germany.

Journal of Biomedical Materials Research. Part A
|November 30, 2013
PubMed
Summary

This study developed novel polycaprolactone (PCL) and Bisphenol A ethoxylated dimethacrylate (BPAEDMA) hydrogel scaffolds for cardiac tissue engineering. The enhanced scaffolds significantly improved cardiac cell attachment, proliferation, and function.

Keywords:
actinincardiac cellselastomerhydrogelnanofiberstroponin

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Polymer Chemistry

Background:

  • Polycaprolactone (PCL) is a biocompatible polymer used in tissue engineering scaffolds.
  • PCL's inherent stiffness and hydrophobicity limit cell attachment and proliferation in cardiac applications.
  • Developing improved scaffolds is crucial for effective cardiac tissue regeneration.

Purpose of the Study:

  • To create and characterize novel electrospun nanofibrous scaffolds combining PCL and Bisphenol A ethoxylated dimethacrylate (BPAEDMA) hydrogel.
  • To evaluate the impact of varying PCL/BPAEDMA ratios on scaffold properties and cardiac cell behavior.
  • To assess the potential of these composite scaffolds for cardiac tissue engineering.

Main Methods:

  • Fabrication of PCL/BPAEDMA composite nanofibrous scaffolds using electrospinning.
  • Characterization of scaffold composition, morphology, and mechanical properties (SEM, FTIR, DMA).
  • In vitro culture of rabbit cardiac cells on scaffolds for 10 days, assessing viability, protein expression, adhesion, and proliferation.

Main Results:

  • Scaffold elastic modulus decreased with increasing BPAEDMA content.
  • Scaffolds with 75 wt% BPAEDMA showed reduced modulus (3.55 MPa) and contact angle (25°).
  • Cardiac cells cultured on 75 wt% BPAEDMA scaffolds exhibited enhanced viability, adhesion, proliferation, and expression of cardiac-specific proteins (troponin, alpha-actinin).

Conclusions:

  • PCL/BPAEDMA composite nanofibrous scaffolds significantly enhance cardiac cell adhesion, proliferation, and function.
  • The developed scaffolds demonstrate potential for clinical applications in cardiac tissue engineering.
  • Optimized composite scaffolds offer a promising alternative to pure PCL for improved cardiac cell integration.