[Effect of different primers on microbial community of activated sludge]

The aim of this study was to investigate the effect of different primers in PCR-DGGE and PCR-RFLP on the analysis of microbial community in activated sludge. 8 pairs of primers were chosen to amplify the variable region of 16S rDNA for PCR-DGGE analysis, while 11 pairs of primers were used to amplify the total length of 16S rDNA for PCR-RFLP analysis. The effect of different primers on the analysis of microbial community in activated sludge was determined by electrophoresis analysis of the PCR products. The microbial community of the activated sludge was different when different primers were used. For PCR-DGGE analysis, the primers B341F/B534R had good amplification results and the bands were excessive; while for PCR-RFLP, the primers 27f/8f and 1500R had good amplification results and the bands digested by the two enzymes had the highest diversity. The primers B341F/B534R and 27f/8f/ 1500R were relatively good for PCR-DGGE and PCR-RFLP, respectively, in the analysis of microbial community in activated sludge.