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Triptolide inhibits histone methyltransferase EZH2 and modulates the expression of its target genes in prostate

Ousman Tamgue1, Cheng-Sen Chai, Lin Hao

  • 1Key Laboratory of Agricultural Molecular Biology, College of Life Science, Northwest A and F University, Yangling, China E-mail : leiming70@hotmail.com, weiyanming2005@aliyun.com.

Asian Pacific Journal of Cancer Prevention : APJCP
|December 3, 2013
PubMed

Insights

Triptolide inhibits prostate cancer cell growth by downregulating EZH2 (enhancer of zeste homolog 2). This suggests EZH2 is a therapeutic target for prostate cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Enhancer of zeste homolog 2 (EZH2) is crucial in prostate cancer (PCa) progression.
  • EZH2 is a potential therapeutic target for PCa.
  • Triptolide exhibits anti-tumor properties, but its mechanism in PCa involving EZH2 was unclear.

Purpose of the Study:

  • To investigate if EZH2 is a therapeutic target for Triptolide in prostate cancer cells.
  • To elucidate the relationship between Triptolide treatment and EZH2 expression in PCa.

Main Methods:

  • Prostate cancer cell lines were treated with Triptolide.
  • EZH2 expression levels were analyzed.
  • The impact of EZH2 overexpression on Triptolide's efficacy was assessed.
  • mRNA levels of EZH2 target genes were quantified.

Main Results:

  • Triptolide suppressed prostate cancer cell proliferation.
  • Triptolide treatment led to decreased EZH2 expression.
  • Overexpression of EZH2 partially reversed Triptolide-induced growth inhibition.
  • Triptolide modulated the expression of EZH2 target genes, including ADRB2, CDH1, CDKN2A, DAB2IP, and cyclin D1.

Conclusions:

  • Triptolide's anti-cancer effect in prostate cancer cells is associated with the downregulation of EZH2.
  • Modulation of EZH2 and its target genes by Triptolide offers a potential therapeutic strategy for PCa.

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