Related Experiment Video

Updated: May 5, 2026

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

8.1K

MicroRNA-183 suppresses retinoblastoma cell growth, invasion and migration by targeting LRP6

Jianwen Wang1, Xiaochun Wang2, Zhongji Li1

  • 1Eye Hospital, First Affiliated Hospital, Harbin Medical University, China.

The FEBS Journal
|December 3, 2013
PubMed

Insights

MicroRNA-183 (miR-183) is downregulated in retinoblastoma (RB). Restoring miR-183 suppresses RB cell growth and invasion by targeting low-density lipoprotein receptor-related protein 6 (LRP6).

Area of Science:

  • Ophthalmology
  • Molecular Biology
  • Oncology

Background:

  • Retinoblastoma (RB) is a pediatric eye cancer.
  • The role of microRNAs (miRNAs) in RB pathogenesis is under investigation.
  • MicroRNA-183 (miR-183) has been implicated in various cancers.

Purpose of the Study:

  • To investigate the role of miR-183 in retinoblastoma.
  • To identify potential targets of miR-183 in RB cells.
  • To explore miR-183 as a therapeutic target for RB.

Main Methods:

  • Quantitative real-time PCR to assess miR-183 expression in RB tissues and cell lines.
  • Cell viability, migration, and invasion assays in RB cell lines with ectopic miR-183 expression.
  • Western blotting and luciferase reporter assays to validate LRP6 as a direct target of miR-183.

Main Results:

  • miR-183 expression was significantly downregulated in retinoblastoma tissues and cell lines compared to normal retinal tissues.
  • Ectopic expression of miR-183 suppressed cell viability, migration, and invasion in RB cell lines (Y79, SO-RB50, WERI-RB1).
  • Low-density lipoprotein receptor-related protein 6 (LRP6) was identified as a direct target of miR-183, and its restoration rescued the inhibitory effects of miR-183.

Conclusions:

  • miR-183 acts as a tumor suppressor in retinoblastoma.
  • miR-183 inhibits RB cell growth, migration, and invasion by downregulating LRP6.
  • Targeting the miR-183/LRP6 pathway may offer a novel therapeutic strategy for retinoblastoma.
Keywords:
LRP6cell growthcell invasionmicroRNA-183retinoblastoma

More Related Videos

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

18.5K
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.1K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.2K
MicroRNAs01:22

MicroRNAs

9.8K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
3.7K
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.5K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
32.1K