Correlation between circulating proteasome activity, total protein and c-reactive protein levels following burn in
E Matuszczak1, M Tylicka2, W Dębek1
1Department of Pediatric Surgery, Medical University of Bialystok, Poland.
Insights
Circulating proteasome activity increases after burn injury in children. This activity correlates with burn severity and may serve as a biomarker for tissue damage.
Area of Science:
- Biochemistry
- Pediatric Surgery
- Biomarkers
Background:
- Burn injuries in children can lead to significant systemic complications.
- Assessing tissue damage and inflammatory responses is crucial for effective management.
- Circulating proteasome activity is a potential indicator of cellular stress and damage.
Purpose of the Study:
- To analyze circulating proteasome (c-proteasome) activity in pediatric burn patients.
- To correlate c-proteasome activity with plasma total protein and C-reactive protein (CRP) levels.
- To investigate the relationship between c-proteasome activity and burn severity.
Main Methods:
- Fifty pediatric patients with scald burns (4-20% TBSA) were studied.
- Plasma proteasome activity was measured at various time points post-injury.
- Activity was assessed using a specific peptide substrate; healthy children served as controls.
Main Results:
- Elevated plasma c-proteasome activity was observed 12-16 hours post-burn in all patient groups.
- C-proteasome activity showed a negative correlation with total protein and a positive correlation with CRP levels.
- C-proteasome activity correlated more strongly with burn severity than CRP levels at 12-16 hours and 3 days post-burn.
Conclusions:
- Circulating 20S proteasome activity increases following burn injury in children.
- Plasma 20S proteasome activity negatively correlates with total protein levels.
- Plasma 20S proteasome activity shows potential as an additional biomarker for tissue damage in pediatric burn patients.
Aim Of The Study:
To characterize burn-induced changes following burn in children by analyzing circulating proteasome (c-proteasome) activity in the plasma in correlation with total protein and c-reactive protein levels in the plasma, and the severity of the burn.
Methods:
Fifty consecutive children scalded by hot water who were managed at the Department of Pediatric Surgery after primarily presenting with burns in 4-20% TBSA were included into the study. The children were aged 9 months up to 14 years (mean age 2.5±1 years). Patients were divided into groups according to the pediatric injury severity score used by American Burns Association. Plasma proteasome activity was assessed using Suc-Leu-Leu-Val-Tyr-AMC peptide substrate, 2-6 h, 12-16 h, 3 days, 5 days, and 7 days after injury. 20 healthy children consecutively admitted for planned inguinal hernia repair served as controls.
Results:
Statistically significant elevation of plasma c-proteasome activity was noted in all groups of burned children 12-16 h after the injury. We found a strong negative correlation of c-proteasome activity with total protein levels, and positive correlation with CRP levels 12-16 h after burn. We also found stronger correlation between c-proteasome activity and severity of burn, than CRP level and severity of burn 12-16 h, and 3 days after the burn. Correlations were statistically significant.
Conclusions:
This study characterized circulating 20S proteasome activity levels after burn. C-proteasome activity elevate after burn and correlate negatively with plasma total protein level, thus plasma 20S proteasome activity could be additional biomarker of tissue damage in burn in pediatric population.
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