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Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...

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Related Experiment Video

Updated: May 5, 2026

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Published on: August 22, 2019

Therapeutic antibody engineering by high efficiency cell screening.

Achim Doerner¹, Laura Rhiel¹, Stefan Zielonka²

¹Protein Engineering and Antibody Technologies, Merck Serono, Merck KGaA, Frankfurter Straße 250, D-64293 Darmstadt, Germany.

|December 3, 2013

Summary

Cell-based screening technologies, particularly yeast surface display, enable efficient isolation of antibodies with desired properties. These methods offer advantages over traditional techniques for therapeutic antibody discovery.

Keywords:

Antibody engineering Cell surface display FACS FC Fab High-throughput library screening Ig PBMC Therapeutic antibody VH VL antigen-binding fragment of an immunoglobulin flow cytometry fluorescence-activated cell sorting heavy chain of an immunoglobulin immunoglobulin light chain of an immunoglobulin mAb monoclonal antibody peripheral blood mononuclear cells scFv single-chain Fv fragment

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Area of Science:

Biotechnology
Immunology
Molecular Biology

Background:

Cell-based screening technologies have emerged for isolating antibodies with specific properties.
These methods involve displaying antibodies on cell surfaces for high-throughput screening.
Yeast surface display combined with fluorescence-activated cell sorting is a powerful technique.

Purpose of the Study:

To review advancements in cell-based antibody screening technologies.
To highlight the advantages of cell-based screening over traditional methods.
To explore emerging techniques for therapeutic antibody isolation.

Main Methods:

Antibody fragments displayed on cell surfaces (e.g., yeast, higher eukaryotic cells).
High-throughput screening using fluorescence-activated cell sorting (FACS).
Utilizing intrinsic eukaryotic protein folding and quality control mechanisms.

Main Results:

Yeast surface display offers advantages over hybridoma and phage display technologies.
Cell-based screening allows for real-time, single-cell analysis.
Eukaryotic hosts facilitate co-selection of expression, stability, and binding functionality.

Conclusions:

Cell-based screening, especially yeast display, is highly effective for antibody isolation.
Emerging technologies using higher eukaryotic cells and B-cell screening show promise.
Combining immunization, B-cell screening, and next-generation sequencing can advance therapeutic antibody development.