Related Experiment Video
Updated: May 5, 2026
Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
Published on: October 20, 2020
Proteomics-based identification of plasma proteins and their association with the host-pathogen interaction in
Abhai Kumar1, Smita Singh2, Suneel Kumar Ahirwar1
1Department of Microbiology, Institute of Medical Science, Banaras Hindu University, Varanasi 221005, India.
Background:
Current diagnostic tests are inadequate to detect typhoid cases, as well as the chronic carrier state, the sole reservoir of Salmonella enterica serovar Typhi. The current study was conducted to find new molecular signatures of pathogen/disease to understand the mechanism behind the host-pathogen interaction in enteric fever.
Methods:
Proteomics-based studies were done to determine the expression of differentially expressed proteins in the plasma of controls, acute typhoid cases, and chronic typhoid carriers. Further, transcriptome-based analysis using reverse-transcriptase PCR (RT-PCR) was done in controls, acute typhoid cases, and chronic typhoid carriers.
Results:
Results showed the upregulation of proprotein convertase subtilisin, furin, haptoglobin, and albumin in the plasma of chronic typhoid carriers. The elevation in mRNA expression of four differentially expressed proteins confirms the changes at the transcriptional level. Further, the increase in albumin and haptoglobin in chronic typhoid carriers shows their role in free radical generation, inflammation, and monocyte cell signaling.
Conclusion:
Through proteomics techniques, this study identified four proteins in the chronic typhoid carrier host that may have a role in the disease pathogenesis of enteric fever.
Insights
This study identified four key proteins in chronic typhoid carriers, offering new molecular signatures for diagnosing Salmonella enterica serovar Typhi infections and understanding host-pathogen interactions in enteric fever.
Area of Science:
- Molecular biology
- Proteomics
- Infectious disease research
Background:
- Current diagnostics fail to detect typhoid fever and chronic Salmonella enterica serovar Typhi carriers.
- The chronic carrier state is the primary reservoir for Salmonella Typhi.
Purpose of the Study:
- Identify novel molecular signatures for pathogen/disease detection.
- Elucidate host-pathogen interactions in enteric fever.
Main Methods:
- Proteomics analysis of plasma from controls, acute typhoid cases, and chronic carriers.
- Transcriptome analysis using reverse-transcriptase PCR (RT-PCR).
Main Results:
- Upregulation of proprotein convertase subtilisin, furin, haptoglobin, and albumin in chronic carriers.
- Elevated mRNA expression confirmed transcriptional changes.
- Increased albumin and haptoglobin linked to free radical generation, inflammation, and monocyte signaling.
Conclusions:
- Proteomics identified four proteins in chronic typhoid carriers potentially involved in disease pathogenesis.
- These findings may lead to improved diagnostic markers for typhoid fever.
More Related Videos
09:43Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP
Published on: October 30, 2016
09:26Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis
Published on: May 23, 2021
