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Related Experiment Videos

Basic fibroblast growth factor induces angiogenesis in vitro.

R Montesano, J D Vassalli, A Baird

    Proceedings of the National Academy of Sciences of the United States of America
    |October 1, 1986
    PubMed
    Summary

    Basic fibroblast growth factor (FGF) stimulates endothelial cells to form blood capillaries in vitro. This process involves cell invasion, tubule formation, and production of urokinase-type plasminogen activator, mimicking in vivo angiogenesis.

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    Area of Science:

    • Cell Biology
    • Biochemistry
    • Angiogenesis Research

    Background:

    • Fibroblast growth factors (FGFs) are known mitogens for endothelial cells.
    • Endothelial cell migration and extracellular matrix invasion are critical for angiogenesis.
    • A functional in vitro model for studying angiogenesis is essential.

    Purpose of the Study:

    • To investigate the role of basic FGF in endothelial cell behavior using an in vitro angiogenesis model.
    • To determine if basic FGF can induce capillary endothelial cells to invade a collagen matrix and form tubules.
    • To assess the effect of basic FGF on urokinase-type plasminogen activator production by endothelial cells.

    Main Methods:

    • Utilized a recently developed in vitro model of angiogenesis.
    • Employed highly purified basic pituitary FGF.

    Related Experiment Videos

  • Cultured capillary endothelial cells in a three-dimensional collagen matrix.
  • Measured tubule formation and urokinase-type plasminogen activator production.

    • Main Results:

    • Basic FGF induced capillary endothelial cells to invade the collagen matrix.
    • Endothelial cells organized into tubules resembling blood capillaries.
    • Basic FGF stimulated endothelial cells to produce urokinase-type plasminogen activator.
    • These findings demonstrate basic FGF's role in endothelial cell migration and invasion.

    Conclusions:

    • Basic FGF promotes key processes of angiogenesis in vitro, including endothelial cell migration, invasion, and tubule formation.
    • Basic FGF stimulates the production of urokinase-type plasminogen activator, a protease involved in neovascularization.
    • The in vitro model effectively recapitulates aspects of in vivo angiogenesis stimulated by basic FGF.