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Related Experiment Video

Updated: May 5, 2026

Establishment of a Human Multiple Myeloma Xenograft Model in the Chicken to Study Tumor Growth, Invasion and Angiogenesis
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Patient-specific 3D microfluidic tissue model for multiple myeloma.

Wenting Zhang1, Woo Y Lee, David S Siegel

  • 11 Chemical Engineering and Materials Science, Stevens Institute of Technology , Hoboken, New Jersey.

Tissue Engineering. Part C, Methods
|December 4, 2013
PubMed
Summary
This summary is machine-generated.

A novel microfluidic platform successfully cultured primary multiple myeloma cells (MMC) by mimicking the bone marrow niche. This advance aids personalized therapeutics and drug evaluation for multiple myeloma.

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Area of Science:

  • Biomedical Engineering
  • Hematology
  • Cancer Biology

Background:

  • Primary multiple myeloma cells (MMC) require the bone marrow microenvironment for survival, posing challenges for in vitro culturing.
  • Existing methods struggle to maintain the viability and biological relevance of primary MMC in laboratory settings.

Purpose of the Study:

  • To develop a novel microfluidic system for culturing primary human multiple myeloma cells (MMC).
  • To emulate the dynamic bone marrow microenvironment, including interactions with osteoblasts (OSB).

Main Methods:

  • Utilized an eight-chamber microfluidic device with a three-dimensional ossified tissue to mimic the tumor niche.
  • Co-cultured primary human bone marrow mononuclear cells from multiple myeloma patients with the hFOB 1.19 osteoblast cell line.
  • Employed optical microscopy for real-time monitoring and flow cytometry for MMC expansion analysis.

Main Results:

  • Mononuclear cells were observed to be drawn towards the osteoblast layer in the microfluidic system.
  • Significant expansion of primary MMC was achieved, with CD138(+) cells showing 2.5-4.6 expansions and CD38(+)CD56(+) cells showing 1-3 expansions over 3 weeks.
  • Demonstrated successful recapitulation of MMC growth within a simulated bone marrow microenvironment.

Conclusions:

  • The microfluidic platform provides a viable method for culturing difficult-to-preserve primary multiple myeloma cells.
  • This approach facilitates personalized therapeutic testing, in vitro drug evaluation, and studying multiple myeloma biology, including drug resistance mechanisms.