Related Experiment Video
Updated: May 5, 2026

Targeted DNA Methylation Analysis by Next-generation Sequencing
Published on: February 24, 2015
Recent advances in the bioanalysis of modified nucleotides in epigenetic studies
Domniki Fragou1, Aikaterini Fragou, Victoria Samanidou
1Laboratory of Forensic Medicine & Toxicology, School of Medicine, Aristotle University of Thessaloniki, 54124, Greece.
Abstract:
Epigenetic alterations, such as DNA methylation, are involved in the pathogenesis of various diseases, the toxicity of diverse agents, the process of aging, the development of stem cells and numerous other mechanisms. DNA methylation is one of the most well-studied epigenetic alterations in mammals. Nevertheless, the scientific interest is now focusing on novel modified nucleotides with potential regulatory roles, such as 5-hydroxymethylcytosine. We currently present and discuss novel bioanalytical strategies developed for the determination of various modified nucleotides in epigenetic studies.
Related Concept Videos
Histone Modification
Histone Modification
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
Epigenetic Regulation
X-chromosome...
Epigenetic Regulation
Biosynthesis of Nucleic Acids

