Heat-induced denaturation and aggregation of actomyosin and myosin from yellowcheek carp during setting
Yuqin Ding1, Ru Liu, Jianhua Rong
1College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China; National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology, Changsha, Hunan 410004, PR China.
Abstract:
Thermal inactivation kinetics of Ca²⁺-ATPase, changes in turbidity and rheological properties of actomyosin and myosin from yellowcheek carp during setting at different temperatures were investigated. Actomyosin and myosin setting at 40-45 °C exhibited greater extent and more rapid Ca²⁺-ATPase inactivation compared to at 25-30 °C. Formation of protein aggregates and three-dimensional network structures of actomyosin and myosin at 25-30 °C was far less than those at 40-45 °C. Thermal stability of actomyosin was higher than that of myosin as revealed by its higher activation energy for the inactivation of Ca²⁺-ATPase. Actomyosin and myosin also exhibited different dynamic rheological properties, especially when the setting temperatures were 40 and 45 °C.
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