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Selective Capture of 5-hydroxymethylcytosine from Genomic DNA

Selective Capture of 5-hydroxymethylcytosine from Genomic DNA

Published on: October 5, 2012

Discrimination between 5-hydroxymethylcytosine and 5-methylcytosine in DNA by selective chemical labeling

Jianlin Hu¹, Yuqi Chen¹, Xiaowei Xu¹

¹College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, PR China.

Bioorganic & Medicinal Chemistry Letters

|December 4, 2013

Abstract:

Detection of DNA damage has been greatly improved following the development of equipment and techniques, however, discrimination between 5-hydroxymethylcytosine (5-hmC) and 5-methylcytosine (5-mC) is still a thorny problem. In the present study, an approach to oxidize and selective label (Ox-Labeling) 5-hmC in native DNA has been reported, which conveniently distinguishes 5-hmC from 5-mC using simple and effective processes.

Keywords:

Chemoselectivity DNA damage Fluorescent dyes Nucleosides Oligodeoxyribonucleotides

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