Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Homologous Recombination02:31

Homologous Recombination

58.9K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
58.9K
Replication in Eukaryotes01:29

Replication in Eukaryotes

15.5K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
15.5K
Replication in Eukaryotes02:31

Replication in Eukaryotes

157.0K
Overview
157.0K
Replication in Eukaryotes01:29

Replication in Eukaryotes

10.7K
10.7K
Replication in Eukaryotes02:31

Replication in Eukaryotes

39.1K
39.1K
Genome Copying Errors02:46

Genome Copying Errors

4.4K
DNA replication is a well-evolved process that copies millions of base pairs with high fidelity during each cell division. Occasionally a wrong base or a long stretch of wrong bases may get added to the daughter strands. If the errors are left unchecked, cells might accumulate several mutations that might endanger their  survival. Therefore, the copying errors are checked and repaired at three levels.
4.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

REV1 Loss Triggers a G2/M Cell-Cycle Arrest Through Dysregulation of Mitotic Regulators.

Genes·2026
Same author

REV1 inhibition enhances trinucleotide repeat mutagenesis.

Open biology·2026
Same author

REV1 inhibition enhances trinucleotide repeat mutagenesis.

bioRxiv : the preprint server for biology·2025
Same author

<i>RPS19</i> and <i>RPL5</i>, the most commonly mutated genes in Diamond Blackfan anemia, impact DNA double-strand break repair.

bioRxiv : the preprint server for biology·2024
Same author

Lead compound profiling for small molecule inhibitors of the REV1-CT/RIR Translesion synthesis Protein-Protein interaction.

Bioorganic & medicinal chemistry·2024
Same author

Evolution of Rev7 interactions in eukaryotic TLS DNA polymerase Polζ.

The Journal of biological chemistry·2023

Related Experiment Video

Updated: May 5, 2026

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis
10:36

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis

Published on: December 21, 2010

10.0K

Replicating damaged DNA in eukaryotes.

Nimrat Chatterjee1, Wolfram Siede

  • 1Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030.

Cold Spring Harbor Perspectives in Biology
|December 4, 2013
PubMed
Summary
This summary is machine-generated.

This review covers how cells maintain genetic stability during DNA replication, focusing on checkpoint responses and error-free damage tolerance mechanisms.

More Related Videos

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
06:59

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Published on: March 31, 2022

1.8K
Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
07:18

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

42.1K

Related Experiment Videos

Last Updated: May 5, 2026

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis
10:36

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis

Published on: December 21, 2010

10.0K
Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
06:59

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Published on: March 31, 2022

1.8K
Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
07:18

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

42.1K

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • DNA damage poses a significant threat to genetic stability during eukaryotic genome replication in S phase.
  • Cellular mechanisms are in place to respond to and mitigate the effects of DNA damage.
  • Understanding these responses is crucial for comprehending genome integrity maintenance.

Purpose of the Study:

  • To provide a general introduction to DNA damage and checkpoint responses during eukaryotic DNA replication.
  • To offer a detailed overview of error-free tolerance mechanisms that handle fork-arresting DNA damage.
  • To synthesize current knowledge on maintaining genetic stability in the face of DNA damage.

Main Methods:

  • Literature review of existing research on DNA damage, checkpoint responses, and tolerance mechanisms.
  • Synthesis of information regarding the molecular pathways involved in genetic stability.
  • Analysis of error-free tolerance strategies in response to DNA replication stress.

Main Results:

  • Eukaryotic cells employ sophisticated checkpoint responses to halt DNA replication upon detecting damage.
  • Error-free tolerance pathways are essential for bypassing or repairing DNA damage without introducing mutations.
  • These mechanisms collectively ensure the accurate duplication of the genome.

Conclusions:

  • Checkpoint activation and error-free damage tolerance are critical for preserving eukaryotic genome stability.
  • Further research into these pathways can illuminate disease mechanisms and therapeutic strategies.
  • Maintaining genetic integrity is a fundamental cellular process vital for organismal health.