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Updated: May 5, 2026

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
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Combining next-generation sequencing and online databases for microsatellite development in non-model organisms.

Ciro Rico1, Eric Normandeau, Anne-Marie Dion-Côté

  • 11] Estación Biológica de Doñana, Consejo Superior de Investigaciones Científicas (EBD, CSIC), C/Américo Vespucio s/n, 41092 Sevilla, Spain [2] School of Marine Studies, University of the South Pacific, Lower Laucala Campus, Suva, Fiji Islands [3] Institut de Biologie Intégrative et des Systèmes (IBIS), Département de Biologie, Université Laval, Pavillon Charles-Eugène-Marchand, Québec G1V 0A6, Canada.

Scientific Reports
|December 4, 2013
PubMed
Summary

Researchers developed 40 new microsatellite markers for whitefish evolution studies using next-generation sequencing data. These polymorphic markers aid in understanding adaptive radiation and demographic history in Coregonus species.

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