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[Macrophage participation in the polyclonal and specific response to T-independent antigen in vitro]
Effect of adherent cells (macrophages on synthesis of total immunoglobulins (Ig) and specific antibody (Ab) in mice spleen cell culture stimulated by Vi-antigen Salmonella typhi has been studied. Ig and Ab in culture medium were determined by ELISA technique 96 hours after introducing the antigen into cell culture. The adsorption of analysed samples and reference antiserum on antigen coated microplates at pH 3.5 results in reducing nonspecific protein adsorption and promotes quantitative analysis Ab synthesized in vitro. The level of Ab produced in antigen stimulated spleen cell culture during 96 hours after antigen introduction is less than 2% of all synthesized Ig. Removal of most macrophages from spleen cell population results in a considerable decrease of specific and polyclonal immune responses. The basal Ig level in spleen cell culture (without antigen influence) does not decrease after removal of macrophages.
Effect of adherent cells (macrophages on synthesis of total immunoglobulins (Ig) and specific antibody (Ab) in mice spleen cell culture stimulated by Vi-antigen Salmonella typhi has been studied. Ig and Ab in culture medium were determined by ELISA technique 96 hours after introducing the antigen into cell culture. The adsorption of analysed samples and reference antiserum on antigen coated microplates at pH 3.5 results in reducing nonspecific protein adsorption and promotes quantitative analysis Ab synthesized in vitro. The level of Ab produced in antigen stimulated spleen cell culture during 96 hours after antigen introduction is less than 2% of all synthesized Ig. Removal of most macrophages from spleen cell population results in a considerable decrease of specific and polyclonal immune responses. The basal Ig level in spleen cell culture (without antigen influence) does not decrease after removal of macrophages.