Use of Multicolor Flow Cytometry for Isolation of Specific Cell Populations Deriving from Differentiated Human
Isabella Mengarelli1, Andrew Fryga, Tiziano Barberi
1Department of Experimental Cardiology, Academic Medical Center, University of Amsterdam, K2-113, Meibergdreef 9, 1105AZ, Amsterdam, The Netherlands, i.mengarelli@amc.uva.nl.
Abstract:
Flow Cytometry-Sorting (FCM-Sorting) is a technique commonly used to identify and isolate specific types of cells from a heterogeneous population of live cells. Here we describe a multicolor flow cytometry technique that uses five distinct cell surface antigens to isolate four live populations with different surface antigen profiles. These profiles were used to help distinguishing between neural and nonneural (the lens) ectoderm derivatives within a highly heterogenous population of differentiating human embryonic stem cells (hESC).
