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Configuration of bioreactors
Dirk E Martens1, Evert J van den End, Mathieu Streefland
1Bioprocess Engineering, Wageningen University, Wageningen, The Netherlands, Dirk.Martens@wur.nl.
Methods in Molecular Biology (Clifton, N.J.)
|December 4, 2013
Summary
This chapter details lab-scale bioreactor configurations and setup for animal cell research. It covers online parameter control, including oxygen and carbon dioxide monitoring, and methods for determining the oxygen transfer coefficient.
Area of Science:
- Biotechnology
- Bioprocess Engineering
- Cell Culture Technology
Background:
- Lab-scale stirred-tank bioreactors (0.2-20 L) are crucial for fundamental animal cell research.
- They are also vital for process development and troubleshooting in large-scale bioproduction.
Purpose of the Study:
- To discuss various bioreactor system configurations and their setup procedures.
- To describe online measurement and control of bioreactor parameters.
- To detail methods for determining the oxygen transfer coefficient.
Main Methods:
- Discussion of different stirred-tank bioreactor configurations.
- Description of bioreactor system setup.
- Explanation of online parameter measurement and control, including PID controller settings.
- Methods for online monitoring of oxygen consumption and carbon dioxide production.
- Procedures for determining the oxygen transfer coefficient.
Main Results:
- Comprehensive overview of lab-scale bioreactor configurations and setup.
- Detailed explanation of online monitoring and control strategies for key bioreactor parameters.
- Established methods for calculating the oxygen transfer coefficient.
Conclusions:
- Effective setup and control of lab-scale bioreactors are essential for successful animal cell research and bioprocess development.
- Online monitoring of parameters like oxygen consumption and carbon dioxide production, alongside accurate oxygen transfer coefficient determination, enhances process understanding and optimization.
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