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Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System
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Efficient processes for protein expression using recombinant baculovirus particles.

Heike Cremer1, Ingrid Bechtold, Marion Mahnke

  • 1NIBR Biologics Center, Novartis Institutes for BioMedical Research, Basel, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)
|December 4, 2013
PubMed
Summary

Baculoviruses are a standard tool for producing recombinant proteins. This chapter details using baculovirus-infected insect cells and in vivo recombination for efficient protein production.

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Virology

Background:

  • Baculoviruses are widely utilized for recombinant protein production in research laboratories.
  • The technology offers a robust system for expressing foreign proteins in insect cells.

Purpose of the Study:

  • To provide a comprehensive overview of baculovirus technology for protein production.
  • To highlight and explain two recent advancements for efficient recombinant protein generation.

Main Methods:

  • Overview of standard baculovirus expression vector system.
  • Detailed explanation of utilizing baculovirus-infected insect cells for protein expression.
  • Description of in vivo recombination-mediated production of recombinant baculoviruses.

Main Results:

  • Baculovirus expression systems are effective for producing a wide range of recombinant proteins.
  • Baculovirus-infected insect cells offer a practical and efficient method for protein production.
  • In vivo recombination provides a streamlined approach for generating recombinant viruses.

Conclusions:

  • Baculovirus technology remains a powerful and adaptable tool for recombinant protein production.
  • Recent developments, including infected insect cells and in vivo recombination, enhance the efficiency and applicability of baculovirus systems.
  • These advancements facilitate broader adoption and optimization of baculovirus-mediated protein expression.