Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recent advances in venom pharmacology reshaping venom-to-drug discovery.

Trends in pharmacological sciences·2026
Same author

Recovering arrhythmic EEG transients from their stochastic interference.

Communications biology·2026
Same author

Retraction notice to "Attenuation of hyperglycemia-associated dyslipidemic, oxidative, cognitive, and inflammatory crises via modulation of neuronal ChEs/NF-κB/COX-2/NOx, and hepatorenal functional deficits by the Tridax procumbens extract" [Biomedicine & Pharmacotherapy 158 (2023) 114114].

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Combining membrane potential and calcium imaging in brain slices using the voltage-sensitive dye ElectroFluor630 and the calcium indicator Calbryte520.

Neurophotonics·2026
Same author

SPARC: a structural pathogenicity algorithm for risk classification of hERG variants.

Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology·2025
Same author

Interventions to increase adherence to antiretroviral therapy in people living with HIV - what works? A systematic review and meta-analysis.

AIDS care·2025

Related Experiment Video

Updated: May 5, 2026

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels
07:17

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Published on: December 13, 2024

2.0K

Combining calcium imaging with other optical techniques.

Marco Canepari1, Dejan Zecevic, Kaspar E Vogt

  • 1Inserm U836, Team 3, BP 170, Grenoble cedex 09, F-38042, France;

Cold Spring Harbor Protocols
|December 4, 2013
PubMed
Summary
This summary is machine-generated.

Combining calcium (Ca2+) imaging with other optical techniques allows for detailed physiological studies. This guide covers essential principles for designing optimal combined optical experiments, enhancing data acquisition and analysis.

More Related Videos

Author Spotlight: Unveiling Neural Coding and Mechanisms of Visual Processing in the Superior Colliculus
10:43

Author Spotlight: Unveiling Neural Coding and Mechanisms of Visual Processing in the Superior Colliculus

Published on: April 21, 2023

3.7K
In vivo Neuronal Calcium Imaging in C. elegans
11:06

In vivo Neuronal Calcium Imaging in C. elegans

Published on: April 10, 2013

24.6K

Related Experiment Videos

Last Updated: May 5, 2026

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels
07:17

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Published on: December 13, 2024

2.0K
Author Spotlight: Unveiling Neural Coding and Mechanisms of Visual Processing in the Superior Colliculus
10:43

Author Spotlight: Unveiling Neural Coding and Mechanisms of Visual Processing in the Superior Colliculus

Published on: April 21, 2023

3.7K
In vivo Neuronal Calcium Imaging in C. elegans
11:06

In vivo Neuronal Calcium Imaging in C. elegans

Published on: April 10, 2013

24.6K

Area of Science:

  • Neuroscience
  • Biophysics
  • Optical Imaging

Background:

  • Calcium (Ca2+) imaging is crucial for high-resolution measurement of Ca2+ signals.
  • Integrating Ca2+ imaging with electrical recordings or membrane potential imaging is vital for correlating electrical and chemical neuronal activity.
  • Stimulating subcellular compartments necessitates photostimulation, further highlighting the need for combined optical techniques.

Purpose of the Study:

  • To introduce fundamental principles for combining Ca2+ imaging with other optical techniques.
  • To provide guidance for researchers aiming to design optimal combined optical experiments.
  • To facilitate the study of complex physiological questions by integrating multiple optical methods.

Main Methods:

  • Discussion of optical design principles for combined imaging setups.
  • Exploration of experimental protocol design for integrated optical measurements.
  • Analysis of optical characteristics and affinity of Ca2+ indicators in conjunction with optical probes.

Main Results:

  • Provides a framework for understanding the interplay between Ca2+ signals and other optical measurements.
  • Highlights key considerations for selecting appropriate Ca2+ indicators and probes.
  • Offers insights into optimizing experimental parameters for combined optical studies.

Conclusions:

  • Combining Ca2+ imaging with other optical techniques significantly enhances the scope of physiological investigations.
  • Understanding the discussed principles is essential for successful experimental design and data interpretation.
  • This approach enables a more comprehensive understanding of cellular and subcellular processes.