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Related Experiment Video

Updated: May 5, 2026

Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons
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Combining Ca2+ and membrane potential imaging in single neurons.

Marco Canepari1, Kaspar E Vogt, Michel De Waard

  • 1Inserm, U836, Team 3, BP 170, Grenoble cedex 09, F-38042, France;

Cold Spring Harbor Protocols
|December 4, 2013
PubMed
Summary

This study presents a method to simultaneously monitor calcium (Ca2+) signals and membrane potential in neurons. This technique allows correlation of electrical and chemical neuronal activity from multiple sites.

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Area of Science:

  • Neuroscience
  • Cellular Biology
  • Biophysics

Background:

  • Understanding neuronal function requires correlating electrical and chemical signals.
  • Simultaneous monitoring of calcium (Ca2+) and membrane potential is crucial for studying neural activity.
  • Traditional methods like microelectrodes have limitations in accessing all neuronal sites.

Purpose of the Study:

  • To describe a protocol for simultaneous monitoring of Ca2+ signals and membrane potential in neurons.
  • To detail the equipment needed for detecting and imaging these dual signals.
  • To discuss potential challenges and the capabilities of this advanced technique.

Main Methods:

  • Loading neurons with two indicators: a Ca2+-sensitive Fura dye and a voltage-sensitive dye (JPW1114).

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  • Utilizing specialized equipment for simultaneous detection and imaging of fluorescence signals from both indicators.
  • Applying the protocol to monitor neuronal activity at multiple locations, including those difficult to access.

    • Main Results:

    • Successful simultaneous detection of Ca2+ transients and membrane potential changes in neurons.
    • Demonstration of correlating electrical and chemical signaling events from various neuronal locations.
    • Identification of the capabilities and limitations of the dual-indicator imaging technique.

    Conclusions:

    • Simultaneous monitoring of Ca2+ and membrane potential provides a powerful tool for neuroscience research.
    • This method enhances the understanding of neuronal function by correlating electrical and chemical signals.
    • The described protocol offers a versatile approach for studying neural activity across multiple sites.