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Related Concept Videos

Embryonic Stem Cells00:57

Embryonic Stem Cells

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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
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Embryonic Stem Cells00:58

Embryonic Stem Cells

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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
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Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

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The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
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Stem Cell Culture01:17

Stem Cell Culture

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Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
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Derivation of Human Embryonic Stem Cells by Immunosurgery
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Human embryonic stem cells: derivation, maintenance and cryopreservation.

Jeoung Eun Lee1, Dong Ryul Lee

  • 1CHA Stem Cell Institute, CHA University, Seoul, Korea.

International Journal of Stem Cells
|December 4, 2013
PubMed
Summary

Human embryonic stem cells (hESCs) offer powerful therapeutic potential but require optimized culture and cryopreservation for clinical use. Establishing a cell bank with diverse hESC lines is crucial for guided differentiation and managing immune responses.

Keywords:
CryopreservationCultureEstablishmentHuman embryonic stem cell

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Area of Science:

  • Stem Cell Biology
  • Regenerative Medicine
  • Immunology

Background:

  • Human embryonic stem cells (hESCs) show promise for treating diseases via cell replacement.
  • Clinical application faces challenges in guided differentiation and immune response control.

Purpose of the Study:

  • To review recent advances in hESC derivation and culture conditions.
  • To introduce cryopreservation methods essential for hESC banking.

Main Methods:

  • Review of scientific literature on hESC derivation and culture.
  • Discussion of various hESC cryopreservation techniques.

Main Results:

  • Optimized culture conditions are vital for genetically stable hESCs.
  • Cryopreservation is key for developing a comprehensive hESC bank.

Conclusions:

  • Advancements in hESC derivation and culture are enabling clinical translation.
  • Effective cryopreservation strategies are necessary for hESC banking to address differentiation and immune variability.