Tissue regeneration and stem cell distribution in adriamycin induced glomerulopathy
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Maha Baligh Zickri1, Marwa Mohamed Abdel Fattah, Hala Gabr Metwally
1Histology Department, Faculty of Medicine, Cairo University.
Background And Objectives:
Glomerulosclerosis develops secondary to various kidney diseases. It was postulated that adriamycin (ADR) induce chronic glomerulopathy. Treatment combinations for one year did not significantly modify renal function in resistant focal segmental glomerulosclerosis (FSGS). Recurrence of FSGS after renal transplantation impacts long-term graft survival and limits access to transplantation. The present study aimed at investigating the relation between the possible therapeutic effect of human mesenchymal stem cells (HMSCs), isolated from cord blood on glomerular damage and their distribution by using ADR induced nephrotoxicity as a model in albino rat.
Methods And Results:
Thirty three male albino rats were divided into control group, ADR group where rats were given single intraperitoneal (IP) injection of 5 mg/kg adriamycin. The rats were sacrificed 10, 20 and 30 days following confirmation of glomerular injury. In stem cell therapy group, rats were injected with HMSCs following confirmation of renal injury and sacrificed 10, 20 and 30 days after HMSCs therapy. Kidney sections were exposed to histological, histochemical, immunohistochemical, morphometric and serological studies. In response to SC therapy multiple Malpighian corpuscles (MC) appeared with patent Bowman's space (Bs) 10 and 20 days following therapy. One month following therapy no remarkable shrunken glomeruli were evident. Glomerular area and serum creatinine were significantly different in ADR group in comparison to control and SC therapy groups.
Conclusions:
ADR induced glomerulosclerosis regressed in response to cord blood HMSC therapy. A reciprocal relation was recorded between the extent of renal regeneration and the distribution of undifferentiated mesenchymal stem cells.
Insights
Human mesenchymal stem cells (HMSCs) from cord blood effectively reversed adriamycin-induced glomerulosclerosis in rats. This study highlights HMSCs as a potential therapy for glomerular damage and kidney disease.
Area of Science:
- Nephrology
- Regenerative Medicine
- Stem Cell Biology
Background:
- Glomerulosclerosis is a common complication of various kidney diseases.
- Adriamycin (ADR) is used to induce chronic glomerulopathy in research models.
- Current treatments for resistant focal segmental glomerulosclerosis (FSGS) have limited efficacy.
Purpose of the Study:
- To investigate the therapeutic potential of human mesenchymal stem cells (HMSCs) from cord blood in an adriamycin-induced nephrotoxicity rat model.
- To evaluate the distribution of HMSCs within the kidney following treatment.
- To assess the impact of HMSCs on glomerular damage.
Main Methods:
- Albino rats were induced with adriamycin (ADR) to model glomerulosclerosis.
- Rats received human mesenchymal stem cells (HMSCs) isolated from cord blood.
- Histological, histochemical, immunohistochemical, morphometric, and serological analyses were performed.
- Kidney sections were examined at 10, 20, and 30 days post-therapy.
Main Results:
- Adriamycin-induced glomerular injury showed significant regression following HMSC therapy.
- Multiple Malpighian corpuscles with patent Bowman's space were observed post-therapy.
- Glomerular area and serum creatinine levels were significantly improved in the HMSC-treated group compared to the ADR group.
Conclusions:
- Human mesenchymal stem cells (HMSCs) from cord blood demonstrate a therapeutic effect on ADR-induced glomerulosclerosis.
- A correlation exists between renal regeneration and the distribution of undifferentiated HMSCs.
- HMSC therapy offers a promising approach for treating glomerular damage.
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