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Effect of stem cell therapy on adriamycin induced tubulointerstitial injury
Maha Baligh Zickri1, Somaya Zaghloul, Mira Farouk
1Histology Department, Faculty of Medicine, Cairo University.
Background And Objectives:
It was postulated that adriamycin (ADR) induce renal tubulointerstitial injury. Clinicians are faced with a challenge in producing response in renal patients and slowing or halting the evolution towards kidney failure. The present study aimed at investigating the relation between the possible therapeutic effect of human mesenchymal stem cells (HMSCs), isolated from cord blood on tubular renal damage and their distribution by using ADR induced nephrotoxicity as a model in albino rat.
Methods And Results:
Thirty three male albino rats were divided into control group, ADR group where rats were given single intraperitoneal (IP) injection of 5 mg/kg adriamycin. The rats were sacrificed 10, 20 and 30 days following confirmation of tubular injury. In stem cell therapy group, rats were injected with HMSCs following confirmation of renal injury and sacrificed 10, 20 and 30 days after HMSCs therapy. Kidney sections were exposed to histological, histochemical, immunohistochemical, morphometric and serological studies. In response to SC therapy, vacuolated cytoplasm, dark nuclei, detached epithelial lining and desquamated nuclei were noticed in few collecting tubules (CT). 10, 20 and 30 days following therapy. The mean count of CT showing desquamated nuclei and mean value of serum creatinine revealed significant difference in ADR group. The mean area% of Prussian blue+ve cells and that of CD105 +ve cells measured in subgroup S1 denoted a significant increase compared to subgroups S2 and S3.
Conclusions:
ADR induced tubulointerstitial damage that regressed in response to cord blood HMSC therapy.
Insights
Human mesenchymal stem cells (HMSCs) from cord blood show therapeutic potential in reversing adriamycin-induced kidney damage in rats. This study highlights HMSCs as a promising treatment for tubulointerstitial injury, aiding kidney function recovery.
Area of Science:
- Nephrology
- Regenerative Medicine
- Stem Cell Biology
Background:
- Adriamycin (ADR) is known to induce renal tubulointerstitial injury.
- Clinicians face challenges in managing renal patients and preventing kidney failure progression.
- Adriamycin-induced nephrotoxicity serves as a model for studying kidney damage and potential therapies.
Purpose of the Study:
- To investigate the therapeutic effect of human mesenchymal stem cells (HMSCs) from cord blood on tubular renal damage.
- To evaluate the distribution of HMSCs in the kidneys following ADR-induced nephrotoxicity.
- To assess the potential of HMSCs in reversing ADR-induced tubulointerstitial injury in an albino rat model.
Main Methods:
- Thirty-three male albino rats were divided into control, ADR-induced nephrotoxicity, and HMSC therapy groups.
- Renal injury was induced using a single intraperitoneal injection of adriamycin (5 mg/kg).
- Histological, histochemical, immunohistochemical, morphometric, and serological analyses were performed on kidney sections at 10, 20, and 30 days post-treatment.
Main Results:
- Adriamycin induced significant tubulointerstitial damage, evidenced by increased serum creatinine and histological changes.
- Human mesenchymal stem cell therapy led to the regression of ADR-induced tubular injury, with reduced vacuolated cytoplasm and desquamated nuclei.
- Immunohistochemical analysis showed increased Prussian blue+ve and CD105+ve cells in the treated group, indicating successful engraftment and therapeutic activity of HMSCs.
Conclusions:
- Adriamycin reliably induces tubulointerstitial damage in the rat kidney.
- Therapy with human mesenchymal stem cells derived from cord blood effectively promotes the regression of this ADR-induced damage.
- Cord blood-derived HMSCs demonstrate significant therapeutic potential for reversing tubulointerstitial injury and preserving kidney function.
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