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Photoacoustic microscopy: a potential new tool for evaluation of angiogenesis inhibitor

Sung-Liang Chen1, Joseph Burnett, Duxin Sun

  • 1Department of Radiology, University of Michigan, Ann Arbor, MI 48109, USA.

Biomedical Optics Express
|December 4, 2013
PubMed

Insights

Photoacoustic microscopy (PAM) effectively evaluates angiogenesis inhibitors in vivo. This technique offers objective, 3D microvascular analysis, complementing traditional optical microscopy for drug development.

Area of Science:

  • Biomedical Optics
  • Medical Imaging
  • Pharmacology

Background:

  • Angiogenesis inhibitors are crucial in cancer therapy.
  • Evaluating their efficacy requires precise microvascular assessment.
  • Current methods like optical microscopy have limitations.

Purpose of the Study:

  • To investigate the feasibility of photoacoustic microscopy (PAM) for evaluating angiogenesis inhibitors.
  • To compare PAM with optical microscopy (OM) for quantifying microvascular density.
  • To explore PAM's potential for 3D microvascular analysis.

Main Methods:

  • Laser-scanning PAM and optical microscopy (OM) were used on chick embryo chorioallantoic membranes (CAMs).
  • Different concentrations of the angiogenesis inhibitor Sunitinib were applied.
  • Microvascular density was quantified and compared between PAM and OM.

Main Results:

  • PAM results showed good agreement with the gold standard OM.
  • Microvascular density correlated with Sunitinib concentration.
  • PAM demonstrated capability for 3D microvascular analysis, unlike 2D OM.

Conclusions:

  • PAM is a feasible and objective tool for evaluating anti-angiogenesis medications.
  • PAM offers advantages over OM, including 3D imaging and functional analysis.
  • PAM shows promise for clinical and preclinical anti-angiogenesis drug assessment.
Keywords:
(170.0180) Microscopy(170.3880) Medical and biological imaging(170.5120) Photoacoustic imaging

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