Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

87.9K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
87.9K
In-situ Hybridization02:31

In-situ Hybridization

9.2K
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
9.2K
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

836
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
836
Sanger Sequencing01:57

Sanger Sequencing

800.8K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
800.8K
DNA Microarrays02:34

DNA Microarrays

16.8K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
16.8K
Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

14.6K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
14.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-Molecule Detection Concepts Enabled by DNA Origami.

Micromachines·2026
Same author

Surface Plasmon Resonance as a Potential Diagnostic Tool for the Detection of CXC Chemokine Receptor 4 (CXCR4) on Extracellular Vesicles.

Biosensors·2026
Same author

SpermFACS: Validation of a Highly Sensitive Sperm Cell Sorting Method for Sexual Assault Casework Analysis.

Analytical chemistry·2026
Same author

Conformation-specific monoclonal antibodies reveal early Tau structural intermediates in Alzheimer's disease.

The Journal of biological chemistry·2026
Same author

Advancing intradermal vaccine delivery: Focus on hollow microneedles and skin models.

Human vaccines & immunotherapeutics·2026
Same author

HematoCARD: A Volumetric Duplicate Dried Blood Spot Collection Cartridge Validated for Therapeutic Drug Monitoring.

Analytical chemistry·2025
Same journal

Aptamer-based CRISPR-Cas12a fluorescent biosensors for serum biomarker detection.

The Analyst·2026
Same journal

A two-step centrifugal microfluidic platform for semi-automated IGRA detection of tuberculosis based on chemiluminescence.

The Analyst·2026
Same journal

On-site rapid identification of animal and plant creams <i>via</i> 2D FeB nanozyme-based colorimetric sensors.

The Analyst·2026
Same journal

Sensitive detection of aflatoxin B1 using a dual-mode fluorescent aptasensor based on cascade signal amplification.

The Analyst·2026
Same journal

Deep learning-enabled microfluidic digital PCR platform for efficient seven-color quantification.

The Analyst·2026
Same journal

Monitoring food spoilage biogenic amines utilizing a blue-emitting fluorescent ionic liquid.

The Analyst·2026
See all related articles

Related Experiment Video

Updated: May 5, 2026

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
10:01

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

Published on: September 27, 2016

7.0K

Emerging technologies for hybridization based single nucleotide polymorphism detection.

Karel Knez1, Dragana Spasic, Kris P F Janssen

  • 1KU Leuven, BIOSYST-MeBioS, Willem de Croylaan 42, Leuven, Belgium. jeroen.lammertyn@biw.kuleuven.be.

The Analyst
|December 4, 2013
PubMed
Summary
This summary is machine-generated.

New methods for detecting single nucleotide polymorphisms (SNPs) are essential for point-of-care diagnostics. This review highlights advances in fast, sensitive SNP detection assays for improved molecular diagnostics.

More Related Videos

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
10:27

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria

Published on: November 10, 2015

12.2K
Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

13.2K

Related Experiment Videos

Last Updated: May 5, 2026

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
10:01

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

Published on: September 27, 2016

7.0K
Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
10:27

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria

Published on: November 10, 2015

12.2K
Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

13.2K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Accurate detection of single nucleotide polymorphisms (SNPs) is critical for developing advanced diagnostic tools.
  • The limited impact of single nucleotide variations on short sequences presents a significant detection challenge.
  • Current methods like PCR and sequencing are labor-intensive and costly for routine diagnostics.

Purpose of the Study:

  • To provide an overview of recent advancements in SNP detection assays.
  • To highlight strategies with potential for point-of-care molecular diagnostics.
  • To address the need for accurate, facile, and sensitive mutation detection methods.

Main Methods:

  • Review of novel detection strategies beyond traditional PCR and sequencing.
  • Focus on methods utilizing specially designed hybridization probes.
  • Exploration of mutation-recognizing enzymes and thermal denaturation techniques.

Main Results:

  • Several new methods offer sensitive mutation detection capabilities.
  • These assays are faster and less labor-intensive than conventional techniques.
  • Advances show strong potential for integration into point-of-care testing.

Conclusions:

  • Emerging SNP detection assays are crucial for the next generation of diagnostics.
  • Novel methods offer improved sensitivity, speed, and cost-effectiveness.
  • These advancements pave the way for wider adoption of point-of-care molecular diagnostics.