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Mononuclear phagocyte activation: activation-associated antigens.
Federation Proceedings
|November 1, 1986
Summary
Mononuclear phagocyte activation involves changes in cell metabolism and membrane composition. The Mo3e antigen
Area of Science:
- Immunology
- Cell Biology
Background:
- Mononuclear phagocyte activation is a complex process involving metabolic and membrane changes.
- Specific cell surface antigens, like Mo3e, are expressed on activated monocytes and macrophages.
Purpose of the Study:
- To investigate the expression and regulation of the Mo3e antigen on activated human mononuclear phagocytes.
- To explore the association between Mo3e expression and monocyte responsiveness to migration inhibitory factor (MIF).
Main Methods:
- Activation of human monocytes and cell lines (U-937, HL-60) using various stimuli (LPS, PMA, etc.).
- Detection of Mo3e antigen expression using specific antibodies.
- Inhibition studies using blockers of protein synthesis, glycosylation, protein kinase, and calcium signaling.
Main Results:
- Mo3e antigen expression is induced on activated human monocytes and specific cell lines.
- Stimulated Mo3e expression is dependent on protein synthesis, N-linked glycosylation, protein kinase activation, and calcium ions.
- Anti-Mo3e antibody blocks the migration inhibitory factor (MIF) response in human monocytes.
Conclusions:
- Mo3e is a marker for activated human mononuclear phagocytes.
- Glycoprotein synthesis, protein kinase pathways, and calcium ions are critical for Mo3e expression.
- Mo3e antigen expression is linked to monocyte responsiveness to MIF.