Related Experiment Video

Updated: Oct 23, 2026

Generation of Human Alloantigen-specific T Cells from Peripheral Blood
09:47

Generation of Human Alloantigen-specific T Cells from Peripheral Blood

Published on: November 21, 2014

Analysis of the biological functions and fine specificity of (T,G)-A--L specific T cell clones

O Axelrod, E Mozes

    Immunobiology
    |August 1, 1986
    PubMed

    Two T cell lines, TPB1 and TPB2, specific for the synthetic polypeptide antigen (T,G)-A--L, were established from (T,G)-A--L primed lymph node cells of C3H.SW(H-2b) mice. Both lines proliferated in the presence of (T,G)-A--L, helped in antibody production in vitro, and secreted IL2 upon stimulation with antigen. The lines differed in the fine specificity of their responses to antigenic stimulation. The line with the broader specificity TPB2 was cloned by limiting dilution, and its derived clones were analyzed. No efficient manifestation of both proliferative activity and helper function could be detected in a single clone. Most of the clones were highly specific to (T,G)-A--L, although 2 of them cross-reacted with the closely related polypeptide (Phe,G)-A--L. Individual clones could trigger B cells for the production of antibodies of the IgM and IgG classes. All helper clones secreted (T,G)-A--L specific helper factors. No correlation was found between efficient secretion of IL2 by the clones and their other biological functions.

    More Related Videos

    Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations00:09

    Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

    Published on: August 22, 2019

    T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing08:59

    T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

    Published on: January 12, 2021

    Related Concept Videos

    Special Features of Adaptive Immunity01:20

    Special Features of Adaptive Immunity

    The adaptive immune system, a crucial component of the overall immune response, offers a highly specialized defense against pathogens. It involves specific cell types and features, enabling it to combat infections effectively and efficiently.
    The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
    T Cell Activation and Clonal Selection01:22

    T Cell Activation and Clonal Selection

    T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
    Naive T cells that have not yet encountered an antigen express two primary CD...
    T Cell Types and Functions01:24

    T Cell Types and Functions

    When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
    Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
    Cytotoxic T Cells-mediated Immune Response01:27

    Cytotoxic T Cells-mediated Immune Response

    Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
    Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...