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Updated: May 5, 2026

OLIgo Mass Profiling OLIMP of Extracellular Polysaccharides
Published on: June 20, 2010
Structure of the oligosaccharides isolated from Prosopis juliflora (Sw.) DC. seed polysaccharide
Himani Bhatia1, P K Gupta, P L Soni
1CMR Institute of Technology, Bangalore 560037, India.
Abstract:
A water soluble polysaccharide isolated from Prosopis juliflora seed was purified and major homogenous fraction obtained by GPC. Complete hydrolysis of the polysaccharide followed by paper chromatography and GLC analysis indicated the presence of d-galactose and d-mannose in the ratio 1:1.10, respectively. Partial hydrolysis of the polysaccharide furnished one hepta-(I), one octa-(II) and nona-(III) saccharides. Hydrolysis of oligosaccharide I, II and III followed by GLC analysis furnished d-galactose and d-mannose in the ratio 3:4, 3:5 and 5:4, respectively. Methylation analysis, periodate oxidation and (1)H NMR spectral studies of oligosaccharides indicated the presence of (1→4) mannose units linked to (1→6) galactose units.
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