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11:49
Quantitative and Temporal Control of Oxygen Microenvironment at the Single Islet Level
Published on: November 17, 2013
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Quantitative and temporal control of oxygen microenvironment at the single islet level
Joe Fu-Jiou Lo1, Yong Wang, Zidong Li
1Department of Mechanical Engineering, University of Michigan-Dearborn.
Journal of Visualized Experiments : Jove
|December 5, 2013
Summary
This study introduces a novel microfluidic device for simultaneous oxygen control and glucose monitoring in pancreatic islets. This technique aids in studying islet hypoxia and preconditioning for transplantation research.
Area of Science:
- Biomedical Engineering
- Cell Biology
- Endocrinology
Background:
- Islet hypoxia is critical for modeling diseases and transplantation outcomes.
- Current methods lack simultaneous oxygen modulation and real-time monitoring of glucose stimulus-secretion coupling.
Purpose of the Study:
- To develop a microfluidic technique for simultaneous oxygen control and monitoring of glucose stimulus-secretion coupling factors in islets.
- To investigate islet preconditioning under intermittent hypoxia.
Main Methods:
- A multilayered microfluidic device using polydimethylsiloxane (PDMS) for integrated aqueous and gas phase modulation.
- Quantitative, sub-minute oxygen modulation (0-21%) controlled by microdispensers.
- Multimodal fluorescence microscopy for real-time monitoring of calcium and KATP channel dynamics.
Main Results:
- The microfluidic device successfully achieved simultaneous oxygenation and monitoring of glucose stimulus-secretion coupling factors.
- Demonstrated quantitative, precise control over oxygen levels for intermittent hypoxia.
- Enabled detailed observation of calcium and KATP channel dynamics during hypoxic events.
Conclusions:
- The developed microfluidic hypoxia technique is a valuable tool for studying islets and ex vivo tissues.
- This simultaneous dual-phase modulation system advances research in islet function and transplantation.
- Facilitates investigation of islet preconditioning and pathophysiological states.
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