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Calcium Phosphate Transfection of Primary Hippocampal Neurons
Published on: November 12, 2013
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Calcium phosphate transfection of primary hippocampal neurons
Miao Sun1, Laura P Bernard, Victoria L Dibona
1Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, Rutgers University.
Journal of Visualized Experiments : Jove
|December 5, 2013
Abstract:
Calcium phosphate precipitation is a convenient and economical method for transfection of cultured cells. With optimization, it is possible to use this method on hard-to-transfect cells like primary neurons. Here we describe our detailed protocol for calcium phosphate transfection of hippocampal neurons cocultured with astroglial cells.
