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MicroRNA-519a demonstrates significant tumour suppressive activity in laryngeal squamous cells by targeting

Z Shen1, G Zhan2, H Deng1

  • 1Department of Otorhinolaryngology (Head and Neck Surgery), Lihuili Hospital of Ningbo University, Ningbo, China.

The Journal of Laryngology and Otology
|December 5, 2013
PubMed
Abstract

Insights

MicroRNA-519a is reduced in laryngeal squamous cell carcinoma and acts as a tumor suppressor by inhibiting HuR. This microRNA (miRNA) may be a therapeutic target for this cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Laryngeal squamous cell carcinoma (LSCC) is a significant global health concern.
  • MicroRNAs (miRNAs) play crucial roles in cancer development and progression.
  • The specific role of microRNA-519a (miR-519a) in LSCC remains to be fully elucidated.

Purpose of the Study:

  • To investigate the expression levels of miR-519a in LSCC tissues.
  • To explore the functional impact of miR-519a on LSCC cell growth and cell cycle.
  • To elucidate the molecular mechanisms underlying miR-519a's function in LSCC, focusing on its interaction with HuR.

Main Methods:

  • Quantitative reverse transcription polymerase chain reaction (qRT-PCR) to measure miR-519a and HuR mRNA levels.
  • MTT assays to assess the effect of miR-519a on LSCC cell proliferation.
  • Western blotting to analyze protein expression of HuR and related genes.
  • Flow cytometry for cell cycle analysis.
  • Pearson correlation analysis to associate miR-519a expression with clinical parameters.

Main Results:

  • miR-519a expression was significantly downregulated in LSCC tissues compared to adjacent non-cancerous tissues.
  • Lower miR-519a expression correlated negatively with poor histological differentiation, advanced tumor-node-metastasis (TNM) stage, lymphatic metastasis, and reduced disease-free survival.
  • Overexpression of miR-519a in LSCC cells inhibited cell growth and induced G2/M phase cell cycle arrest.
  • miR-519a suppressed HuR protein expression without altering its mRNA levels, suggesting post-transcriptional regulation.

Conclusions:

  • miR-519a functions as a tumor suppressor in LSCC.
  • The tumor-suppressive role of miR-519a is mediated, at least in part, by the downregulation of HuR.
  • miR-519a holds potential as a diagnostic biomarker and a therapeutic target for laryngeal squamous cell carcinoma.

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