In vitro dissociation-recombination of malate dehydrogenase subunits in Corydalis solida
A H Nagy1, M O Siddiqui, Z G Kocsis
1Department of Genetics, Eötvös Loránd University, Budapest, Hungary.
Abstract:
Two allelic forms of NAD specific malate dehydrogenase were found in samples of a wild population of Corydalis solida. The dimeric nature and the origin of the heterodimeric form has been demonstrated by in vitro dissociation and recombination of the subunits detected by subsequent electrophoresis. The method is applicable for polyacrylamide gel electrophoresis of crude leaf extracts of individual MDH isozyme forms.
