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Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
Published on: December 23, 2017
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Nodulin gene expression during soybean (Glycine max) nodule development.
T Gloudemans1, S de Vries, H J Bussink
1Department of Molecular Biology, Agricultural University, De Dreijen 11, 6703 BC, Wageningen, The Netherlands.
Plant Molecular Biology
|December 5, 2013
Summary
Soybean nodule development involves over 20 nodulin genes, categorized into early (Class A) and late (Class B) expressing groups. The nif H,DK region is not essential for nodulin gene induction.
Area of Science:
- Plant molecular biology
- Legume symbiosis
- Biochemistry
Background:
- Soybean nodule development is a complex process involving coordinated gene expression.
- Nodulin genes play crucial roles in establishing and maintaining symbiotic relationships between soybeans and Bradyrhizobium japonicum.
- Understanding the temporal regulation of nodulin genes is key to optimizing nitrogen fixation.
Purpose of the Study:
- To investigate the temporal expression patterns of nodulin genes during soybean nodule development.
- To determine the role of the nif H,DK region in nodulin gene induction.
- To analyze the impact of specific Bradyrhizobium japonicum mutants on nodulin gene expression.
Main Methods:
- Isolation of total RNA from soybean nodules at different developmental stages.
- Analysis of in vitro translation products using two-dimensional gel electrophoresis.
- Utilizing Bradyrhizobium japonicum nod (+) fix (-) mutants to study nodulin gene induction.
Main Results:
- Over 20 nodulin mRNAs were detected, with distinct temporal expression patterns.
- Nodulin genes were classified into Class A (early, transient) and Class B (late, sustained) based on expression timing.
- Nodules induced by nif H,DK deletion mutants showed normal expression of all nodulin genes.
- In nodules induced by mutant HS124, most nodulin genes were expressed, but 5 Class B genes were not.
Conclusions:
- Soybean nodulin gene expression is temporally regulated, with distinct classes appearing at specific developmental stages.
- The nif H,DK region of Bradyrhizobium japonicum is not required for nodulin gene induction.
- Specific bacterial mutants can affect nodulin gene expression, highlighting the intricate interplay between symbionts.
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