Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pollination and Flower Structure02:40

Pollination and Flower Structure

62.6K
Flowers are the reproductive, seed-producing structures of angiosperms. Typically, flowers consist of sepals, petals, stamens, and carpels. Sepals and petals are the vegetative flower organs. Stamens and carpels are the reproductive organs.  
62.6K
Microbe-Plant Interactions01:09

Microbe-Plant Interactions

140
Microbe-plant interactions represent a dynamic spectrum of associations shaped by intricate chemical signaling. These interactions can be neutral, beneficial, or detrimental, and profoundly influence plant physiology, growth, and ecosystem function. The plant microbiome, comprising bacteria, fungi, archaea, protists, and viruses, plays a pivotal role in mediating these effects through surface colonization, internal colonization, or systemic symbiosis.Mutualistic associations, particularly with...
140
Dihybrid Crosses01:18

Dihybrid Crosses

61.3K
Overview
61.3K
Cell Signaling in Plants01:25

Cell Signaling in Plants

4.5K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
4.5K
The Angiosperm Life Cycle02:39

The Angiosperm Life Cycle

62.6K
Plants have a life cycle split between two multicellular stages: a haploid stage—with cells containing one set of chromosomes—and a diploid stage—with cells containing two sets of chromosomes. The haploid stage is the gamete-producing gametophyte, and the diploid stage is the spore-producing sporophyte.
62.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Anther dehiscence in Lycopersicon esculentum: II. Water relations.

The New phytologist·2021
Same author

The role of the exine coating in pollen-stigma interactions in Brassica oleracea L.

The New phytologist·2021
Same author

Pollination in species with dry stigmas: the nature of the early stigmatic response and the pathway taken by pollen tubes.

The New phytologist·2021
Same author

Identification of pollen components regulating pollination-specific responses in the stigmatic papillae of Brassica oleracea.

The New phytologist·2018
Same author

Time relationships of sporopollenin synthesis associated with tapetum and microspores in Lilium.

Planta·2014
Same author

The rôle of the tapetum in the formation of sporopollenin-containing structures during microsporogenesis in Pinus banksiana.

Planta·2014

Related Experiment Video

Updated: May 5, 2026

Author Spotlight: A High-Resolution, Single-Grain, In Vivo Pollen Hydration Bioassay for Arabidopsis thaliana
07:07

Author Spotlight: A High-Resolution, Single-Grain, In Vivo Pollen Hydration Bioassay for Arabidopsis thaliana

Published on: June 30, 2023

3.0K

Pollen stigma interactions in Brassica oleracea.

I N Roberts1, A D Stead, D J Ockendon

  • 1Department of Botany, Plant Science Laboratories, The University of Reading, Whiteknights, Reading, England.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik
|December 5, 2013
PubMed
Summary

Researchers elucidated self-incompatibility in Brassica, identifying key molecules on pollen and stigma surfaces. Recognition triggers a complex inhibiting water supply, preventing incompatible pollen germination and ensuring successful fertilization.

More Related Videos

Collection and Identification of Pollen from Honey Bee Colonies
08:11

Collection and Identification of Pollen from Honey Bee Colonies

Published on: January 19, 2021

8.9K
Determination of Self- and Inter-incompatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses
08:08

Determination of Self- and Inter-incompatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses

Published on: June 16, 2020

6.9K

Related Experiment Videos

Last Updated: May 5, 2026

Author Spotlight: A High-Resolution, Single-Grain, In Vivo Pollen Hydration Bioassay for Arabidopsis thaliana
07:07

Author Spotlight: A High-Resolution, Single-Grain, In Vivo Pollen Hydration Bioassay for Arabidopsis thaliana

Published on: June 30, 2023

3.0K
Collection and Identification of Pollen from Honey Bee Colonies
08:11

Collection and Identification of Pollen from Honey Bee Colonies

Published on: January 19, 2021

8.9K
Determination of Self- and Inter-incompatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses
08:08

Determination of Self- and Inter-incompatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses

Published on: June 16, 2020

6.9K

Area of Science:

  • Plant reproductive biology
  • Molecular genetics
  • Biochemistry

Background:

  • Self-incompatibility (SI) is a crucial genetic mechanism preventing self-fertilization in many flowering plants, including Brassica species.
  • Understanding SI is vital for crop breeding and improving agricultural yields.
  • Previous research has identified some components of the SI system, but the precise molecular interactions remain under investigation.

Purpose of the Study:

  • To detail the molecular mechanisms underlying self-incompatibility in Brassica.
  • To identify the specific molecules involved in pollen-stigma recognition.
  • To elucidate the sequence of events leading to compatible and incompatible interactions.

Main Methods:

  • Detailed characterization of pollen and stigma surface molecules.
  • Analysis of molecular interactions during pollination, adhesion, hydration, germination, and tube growth.
  • Observation and description of incompatible pollen rejection at various stages.

Main Results:

  • Identified specific recognition molecules on both pollen and stigma surfaces.
  • Outlined a detailed chronological sequence of events in pollen-stigma interactions.
  • Characterized the distinct features of incompatible pollen rejection.
  • Proposed a model where recognition triggers a complex inhibiting water supply to incompatible pollen.

Conclusions:

  • The recognition of pollen coating proteins by stigma pellicle molecules initiates a cascade response.
  • This initial molecular interaction forms a complex that inhibits water uptake by incompatible pollen grains.
  • All observed manifestations of self-incompatibility are downstream consequences of this primary water-inhibition response.