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Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
Published on: October 19, 2018
Subcellular localization of acid proteinase in barley mesophyll protoplasts
U Heck1, E Martinoia, P Matile
1Laboratorium für Pflanzenphysiologie der E.T.H. Zürich, Institut für Allgemeine Botanik, Sonneggstraße 5, CH-8006, Zürich, Switzerland.
Abstract:
Chloroplasts prepared from lysed protoplasts of barley mesophyll contain 2-8% of the total acid proteinase activity. This residual activity is not associated with intact chloroplasts isolated by means of density gradient centrifugation. Vacuoles isolated from lysed protoplasts contain 80-85% of the total acid proteinase activity, indicating that the enzyme(s) which is presumably responsible for the degradation of chloroplastic proteins is located largely in the central vacuoles of mesophyll cells.
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