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[Sequences containing the Alu elements from a human fibroblast cDNA clone library: nucleotide sequence and expression
Molekuliarnaia Biologiia
|September 1, 1986
Summary
Researchers identified Alu elements within human messenger RNA (mRNA) transcripts, specifically at the 3' end. These transcripts were detected in both normal and tumor tissues, suggesting a functional role for these repetitive DNA sequences.
Area of Science:
- Molecular Biology
- Genetics
- Human Genomics
Context:
- Investigating the structure and function of repetitive DNA elements in the human genome.
- Characterizing expressed sequences derived from human fibroblast cDNA libraries.
Purpose:
- To determine the location and orientation of Alu repeats within human mRNA.
- To analyze the expression of Alu-containing transcripts in various human tissues.
Summary:
- Alu-containing cDNA clones were isolated and sequenced, revealing Alu repeats at the presumed 3'-end of mRNA.
- Northern blot analysis confirmed the presence of Alu-homologous transcripts (7-12S) in human tissues, predominantly in a single orientation.
- This orientation contained functional regions, including a promoter for RNA polymerase III.
Impact:
- Provides insights into the transcriptional activity and potential regulatory roles of Alu elements in human gene expression.
- Highlights the presence of Alu-transcripts in both normal and tumor tissues, suggesting broader biological relevance.
- Characterizes a specific orientation of Alu repeats with potential functional implications for RNA polymerase III.