Related Experiment Video
Updated: May 5, 2026

A Highly Scalable Approach to Perform Ecological Surveys of Selfing Caenorhabditis Nematodes
Published on: March 1, 2022
Unique repetitive sequences of 170 bp inChlorella
H Hirano1, Y Takeda, T Iwamura
1Research Institute for Biochemical Regulation, Faculty of Agriculture, Nagoya University, Furo-cho, Chikusa-ku, 464, Nagoya, Japan.
Abstract:
Cot analysis ofChlorella DNA revealed that the genome of the unicellular green alga contained a small amount of repetitive sequences (at most 15% of the total DNA). Short repetitive sequences (SRS) of 170 bp produced by enzymatic digestion of algal DNA with eitherHaeIII,HinfI, orPstI, were found by polyacrylamide gel electrophoresis, and their copy number was estimated to be a few hundred (about 2% of the total repetitive sequences). All three members showed high sequence homology and could be be unified into one family, 'HaeIII family'. The family was divided further into two subfamilies,HinfI- (HaeIII-andHinfI-SRS) andPstI-(PstI-SRS) subfamilies, based on small sequence differences among the members. TheHaeIII family had characteristic structural features, including a considerable number of small unique sequence units (purine-CC) and both direct and inverted repeats, and were organized in tandem arrays in the genome.
More Related Videos
Related Concept Videos
Next-generation Sequencing
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Cis-regulatory Sequences
Cis-regulatory Sequences
Diversity of Protists I
Maxam-Gilbert Sequencing
Challenges of the Maxam-Gilbert Method
The...
Genomic DNA in Eukaryotes

