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Development and Validation of RP-HPLC Method for Simultaneous Estimation of Picroside I, Plumbagin, and
Meenakshi S Akhade1, Poonam A Agrawal, K S Laddha
1Medicinal Natural Products Research Laboratory, Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Matunga (E), Mumbai-400 019, India.
Indian Journal of Pharmaceutical Sciences
|December 5, 2013
Summary
A new reversed-phase high-performance liquid chromatography method accurately quantifies picroside I, plumbagin, and Z-guggulsterone in polyherbal formulations. This validated method ensures reliable quality control for herbal medicines.
Area of Science:
- Analytical Chemistry
- Pharmacognosy
- Phytochemistry
Background:
- Polyherbal formulations containing Picrorhiza kurroa, Plumbago zeylanica, and Commiphora wightii are widely used.
- Standardized quality control of these complex mixtures is essential for therapeutic efficacy and safety.
- Accurate quantification of key phytochemical markers is crucial for ensuring consistency.
Purpose of the Study:
- To develop and validate a reversed-phase high-performance liquid chromatography (RP-HPLC) method.
- To simultaneously estimate three key active compounds: picroside I, plumbagin, and Z-guggulsterone.
- To establish a reliable quality control method for polyherbal formulations.
Main Methods:
- Utilized a C18 column with a gradient mobile phase of acetonitrile and 0.1% orthophosphoric acid in water.
- Employed ultraviolet (UV) detection at 255 nm for sensitive compound detection.
- Validated the method according to International Conference on Harmonization (ICH) guidelines for accuracy, precision, linearity, specificity, and sensitivity.
Main Results:
- The developed RP-HPLC method demonstrated excellent specificity, accuracy, precision, reliability, and reproducibility.
- Achieved good linear correlation coefficients (r² > 0.900) for all three analytes.
- Reported low limits of detection (LOD) and quantification (LOQ), with high recovery rates (100.21% for picroside I, 102.5% for plumbagin, 103.84% for Z-guggulsterone) and low relative standard deviations (<3.0%).
Conclusions:
- The validated RP-HPLC method is suitable for the simultaneous quantitative analysis of picroside I, plumbagin, and Z-guggulsterone.
- The method provides well-resolved peaks, enabling efficient analysis in polyherbal tablet dosage forms.
- This rapid and cost-effective method serves as a valuable quality control tool for routine analysis of these herbal ingredients.
