Related Experiment Video

Updated: May 5, 2026

In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
05:57

In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells

Published on: May 16, 2011

25.5K

The Campylobacter jejuni Cj0268c protein is required for adhesion and invasion in vitro

A Malik Tareen1, Carsten G K Lüder, Andreas E Zautner

  • 1University Medical Center Göttingen, Institute for Medical Microbiology, Göttingen, Germany.

Plos One
|December 5, 2013
PubMed

Insights

A newly identified Campylobacter jejuni protein, Cj0268c, significantly enhances bacterial adherence to host cells. This protein also contributes to bacterial cell wall stability, aiding in invasion processes.

Area of Science:

  • Microbiology
  • Bacterial Pathogenesis
  • Molecular Biology

Background:

  • Campylobacter jejuni adheres to host cells via specific pathogen proteins.
  • Identifying novel adherence factors is crucial for understanding bacterial invasion.

Purpose of the Study:

  • To identify and characterize novel Campylobacter jejuni proteins involved in host cell adherence and invasion.
  • To elucidate the role of the Cj0268c protein in the pathogenesis of C. jejuni.

Main Methods:

  • Screening of a transposon mutant library of C. jejuni for invasion-deficient phenotypes.
  • In vitro characterization of Cj0268c mutant, complemented strains, and parental strains.
  • Heterologous expression of Cj0268c in E. coli for adherence assays.
  • Flow cytometry to determine protein localization.
  • Triton X-100 resistance assays to assess cell wall stability.

Main Results:

  • A C. jejuni mutant with transposon insertion in cj0268c exhibited a significant defect in invasion and adherence to Caco2 cells.
  • Cj0268c is sufficient to mediate adherence to host cells when expressed heterologously in E. coli.
  • Cj0268c localizes to the periplasmic space and contributes to bacterial cell wall stability.
  • The cj0268c gene is ubiquitous in C. jejuni isolates and does not correlate with specific clonal groups.

Conclusions:

  • Cj0268c is a novel Campylobacter jejuni protein that mediates adherence to host cells and enhances invasion.
  • Cj0268c plays a role in maintaining bacterial cell wall integrity.
  • The findings provide new insights into the molecular mechanisms of C. jejuni pathogenesis.

More Related Videos

Enteric Bacterial Invasion Of Intestinal Epithelial Cells In Vitro Is Dramatically Enhanced Using a Vertical Diffusion Chamber Model10:52

Enteric Bacterial Invasion Of Intestinal Epithelial Cells In Vitro Is Dramatically Enhanced Using a Vertical Diffusion Chamber Model

Published on: October 22, 2013

13.3K
Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain14:25

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

Published on: December 12, 2017

17.6K

Related Concept Videos

Adherens Junctions01:24

Adherens Junctions

Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types –  adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as  epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as  heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
5.8K
Bacterial Translocation and Protein Secretion01:26

Bacterial Translocation and Protein Secretion

Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
1.1K
Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
3.3K
Chemotaxis in E. coli01:27

Chemotaxis in E. coli

Chemotaxis in Escherichia coli is a sensory-driven motility mechanism that enables bacteria to navigate chemical gradients, moving toward beneficial environments while avoiding harmful conditions. This process relies on a signal transduction system integrating external chemical cues with flagellar motor control.Chemoreceptors and Signal DetectionE. coli detects chemical gradients through methyl-accepting chemotaxis proteins (MCPs), which are membrane-bound chemoreceptors that sense attractants...
1.4K
Regulation of Bacterial Virulence01:28

Regulation of Bacterial Virulence

Pathogenic bacteria employ a range of regulatory mechanisms to modulate the expression of virulence genes in response to environmental and host-derived signals. These mechanisms ensure that virulence factors are expressed only under favorable conditions, thereby optimizing infection and survival strategies.Mechanisms of Virulence RegulationKey regulatory strategies include:Two-Component Systems: These consist of a membrane-bound sensor kinase and a cytoplasmic response regulator. Environmental...
76
Cytoskeletal Proteins in Bacteria01:29

Cytoskeletal Proteins in Bacteria

Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
3.5K